Metagenomic analysis of microbial community structure, antibiotic resistance genes, and virulence factors of ticks captured at Wenzhou port, Zhejiang province, China

XU Xue-lian, HAN A-xiang, YE Shi-qing, GUAN Wan-chun, LOU Yong-liang

doi:10.11853/j.issn.1003.8280.2021.06.021

Chinese Journal of Vector Biology and Control >

2021 , Vol. 32 >Issue 6: 763 - 771

DOI: https://doi.org/10.11853/j.issn.1003.8280.2021.06.021

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Metagenomic analysis of microbial community structure, antibiotic resistance genes, and virulence factors of ticks captured at Wenzhou port, Zhejiang province, China

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Wenzhou Key Laboratory of Sanitary Microbiology, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Received date: 2021-05-11

Online published: 2021-12-15

Supported by

Supported by the National Science and Technology Major Project (No. 2018ZX10201001-009)

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Abstract

Objective To investigate the microbial community structure, antibiotic resistance genes (ARGs), and virulence factors by the metagenomic technique in ticks captured from wet-salted cow hides imported from South Africa at a port in Wenzhou, Zhejiang province, China. Methods We randomly divided the captured ticks into 5 groups, extracted DNA using HiPure Bacterial DNA Kits, and used high-throughput sequencing to analyze the microbial community structure, pathogenic bacteria, ARGs, and virulence factors of the ticks. Results The 5 samples had similar microbial community structure. At the phylum level, Firmicutes and Proteobacteria were the dominant phyla, and Firmicutes was the most abundant, accounting for more than 66.00% of the total phyla. At the genus level, Streptococcus (>55.30%) and Bacillus (>11.20%) were the dominant genera. All the tick samples were positive for St. mutans, Ba. cereus, Salmonella enterica, and St. pneumoniae, accounting for more than 71.00% of the total species annotated. Sixteen ARGs were identified in the tick samples, including Acr, bacA, mdtK, arnA, and mdtH, and Acr had the highest abundance (30.19%), followed by bacA (20.75%). These ARGs mediated resistance to antibiotics such as macrolides, aminoglycosides, β-lactamases, glycylcycline, and acriflavine. A total of 3 923 virulence genes were found in the tick samples through annotation with the virulence factor database, which were mainly related to adherence (22.66%), secretion system (13.97%), and invasion (13.56%). Conclusion The imported ticks carry various species of pathogens, including highly harmful pathogens such as Sa. enterica, and harbor numerous ARGs and virulence factors. Relevant authorities and frontline workers should pay attention to preventing imported tick-borne diseases and taking effective treatment measures to avoid affecting public health in China.

Key words： Antibiotic resistance gene; Microbial community structure; Metagenomics; Tick; Virulence factor

Cite this article

XU Xue-lian, HAN A-xiang, YE Shi-qing, GUAN Wan-chun, LOU Yong-liang . Metagenomic analysis of microbial community structure, antibiotic resistance genes, and virulence factors of ticks captured at Wenzhou port, Zhejiang province, China[J]. Chinese Journal of Vector Biology and Control, 2021 , 32(6) : 763 -771 . DOI: 10.11853/j.issn.1003.8280.2021.06.021

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