Objective To investigate the expression characteristics of the juvenile hormone receptor gene CpMet of Culex pipiens pallens and its regulatory effects on reproduction. Methods Real-time quantitative PCR was used to determine the expression characteristics of the CpMet gene in different developmental stages of Cx. pipiens pallens, in different tissues of adult mosquitoes, and after hormone treatment. RNA interference technology was used to analyze the role of the CpMet gene in regulating the reproduction of female adult Cx. pipiens pallens. All data were expressed as the mean ±standard error of the mean and analyzed by SPSS 13.0 softwere. Results CpMet was expressed in all developmental stages of Cx. pipiens pallens. The expression level of CpMet was the highest in the egg stage, followed by the pupal and adult stages, and was very low in the larval stage. CpMet had a high expression level in the ovary of female adult mosquitoes, followed by the fat body. After treatment with the juvenile hormone analogue methoprene for 6 h, 12 h, and 24 h, the expression of CpMet was up-regulated by 1.27 times, 4.15 times, and 1.26 times in female adult mosquitoes, respectively, indicating that CpMet was positively regulated by juvenile hormone. After treating mosquito pupae with ecdysone 20E for 24 h, the expression of CpMet was increased sharply, indicating that 20E could induce CpMet gene expression. At 48 h after dsCpMet injection, the mRNA expression of CpMet was significantly decreased by 86.29%. In the egg masses produced by female mosquitoes with dsCpMet injection, the mean number of eggs dropped by 49.89%, and the hatching rate of eggs dropped to 73.36%. Conclusion Interfering with CpMet gene expression can significantly reduce the egg production of female Cx. pipiens pallens mosquitoes, indicating that CpMet can regulate the reproduction of Cx. pipiens pallens and can be used as a potential target for mosquito control.
ZHOU Chang-yin, GUAN Qing-qing, DAI Yu-qi, LIU Hong-xia, QIAN Kun
. Expression characterization and functional analysis of juvenile hormone receptor gene CpMet in Culex pipiens pallens[J]. Chinese Journal of Vector Biology and Control, 2021
, 32(6)
: 680
-685
.
DOI: 10.11853/j.issn.1003.8280.2021.06.005
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