Cloning and bioinformatics analysis of voltage-gated sodium channel gene of <i>Aedes albopictus</i>

ZHOU Xin-xin, LI Fen, DUAN Wen-bo, MA Xin-ran, SONG Xiu-ping, WU Shao-ying, MENG Feng-xia

doi:10.11853/j.issn.1003.8280.2021.06.004

Chinese Journal of Vector Biology and Control >

2021 , Vol. 32 >Issue 6: 672 - 679

DOI: https://doi.org/10.11853/j.issn.1003.8280.2021.06.004

Experimental Study

Cloning and bioinformatics analysis of voltage-gated sodium channel gene of Aedes albopictus

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1. Key Laboratory of Infectious Disease Prevention and Control, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2. The Innovation Team of Research, Development, and Application of Key Technologies for Safe Production of the Tropical Crops, Hainan University, Haikou, Hainan 570228, China

Received date: 2021-07-05

Online published: 2021-12-15

Supported by

Supported by the National Science and Technology Major Project of China (No. 2018ZX10101002-002, 2017ZX10303404002005), National Natural Science Foundation of China (No. 31960539, 31901894) and the Hainan University Foundation (No. KYQD-ZR-1963, KYQD-ZR-1951)

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Abstract

Objective Voltage-gated sodium channel (VGSC) is a specific target-site for pyrethroid insecticides. To obtain the cDNA sequence of the VGSC gene of Aedes albopictus and analyze its molecular characteristics using the bioinformatics techniques, and to provide a basis for investigating the molecular mechanism of Ae. albopictus developing target-site resistance to pyrethroid insecticides. Methods We cloned the VGSC gene sequence of Ae. albopictus by reverse transcription-polymerase chain reaction, and used bioinformatics techniques to analyze its biological features. Results The total length of the VGSC gene of Ae. albopictus was 6 357 bp, and encoded 2 118 amino acids, with an isoelectric point of 5.06 and a molecular weight of the protein of 237×10³. Three alternative splicing sites (m, h, and d) and six RNA editing sites were found. The basic local alignment search tool found that the VGSC genes of Ae. albopictus and Ae. aegypti were highly homologous, with the similarity of 96.30%, and the cloned VGSC gene sequence of Ae. albopictus had the typical characteristics of the sodium channel of insects. Conclusion The full-length cDNA sequence of the VGSC gene of Ae. albopictus is cloned and its bioinformatics characteristics are analyzed, which is helpful in clarifying resistance mechanism and developing new targets, especially for the molecular detection of target-site resistance to pyrethroid insecticides.

Key words： Aedes albopictus; Sodium channel gene; Clone; Bioinformatics

Cite this article

ZHOU Xin-xin, LI Fen, DUAN Wen-bo, MA Xin-ran, SONG Xiu-ping, WU Shao-ying, MENG Feng-xia . Cloning and bioinformatics analysis of voltage-gated sodium channel gene of Aedes albopictus[J]. Chinese Journal of Vector Biology and Control, 2021 , 32(6) : 672 -679 . DOI: 10.11853/j.issn.1003.8280.2021.06.004

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