Objective To evaluate the effects of the Metarhizium anisopliae CQMa421 bait in controlling Blattella germanica in both laboratory and field settings, and to provide a reference for the application of fungal insecticides in the control of hygiene pests. Methods The M. anisopliae CQMa421 baits at different concentrations were prepared, and their effects on the population density of B. germanica were measured in the laboratory and field settings. SPSS 25.0 software was used to analyze mortality, median lethal time (LT50), median lethal concentration (LC50), and 90% lethal concentration (LC90) to evaluate the cockroach-killing effects of the M. anisopliae CQMa421 baits. The chi-square test was used to compare mortality between groups. Results The laboratory test showed that after feeding B. germanica for 12 days with the M. anisopliae CQMa421 bait at 1×108 spores/g, LT50was (6.85±0.24) days. In the field test, after placing the M. anisopliae CQMa421 bait at 1×108 spores/g (1 box/per 2.5 m2) for 30 days, the relative density of B. germanica decreased by 93.22%. Conclusion The M. anisopliae CQMa421 bait at 1×108 spores/g can effectively control the population density of B. germanica.
HE Ya-ming, JI Heng-qing, PENG Guo-xiong, XIAO Han-sen, TU Tao-tian, ZHANG Ying, YU Zhan, LIU Zong-ling, WANG Xiu-juan, LI Ming
. Efficacy of Metarhizium anisopliae CQMa421 bait against cockroaches: A laboratory and field study[J]. Chinese Journal of Vector Biology and Control, 2021
, 32(5)
: 633
-636
.
DOI: 10.11853/j.issn.1003.8280.2021.05.025
[1] 郭慧,王高明,鲁怡欣,等. 武汉市2007-2016年蜚蠊监测结果分析[J]. 中国媒介生物学及控制杂志,2018,29(2):168-171. DOI:10.11853/j.issn.1003.8280.2018.02.013.Guo H,Wang GM,Lu YX,et al. Analysis of cockroach surveillance in Wuhan city from 2007 to 2016[J]. Chin J Vector Biol Control,2018,29(2):168-171. DOI:10.11853/j.issn.1003. 8280.2018.02.013.
[2] 张家勇,王纯玉,丁俊,等. 2014-2016年辽宁省蟑螂监测分析[J]. 中华卫生杀虫药械,2019,25(4):338-342. DOI:10.19821/j.1671-2781.2019.01.013.Zhang JY,Wang CY,Ding J,et al. Surveillance on cockroaches in Liaoning province from 2014 to 2016[J]. Chin J Hyg Insect Equip,2019,25(4):338-342. DOI:10.19821/j.1671-2781.2019. 01.013.
[3] Sheih A,Parks WC,Ziegler SF. GM-CSF produced by the airway epithelium is required for sensitization to cockroach allergen[J]. Mucosal Immunol,2017,10(3):705-715. DOI:10.1038/mi.2016.90.
[4] Brenner RJ. Economics and medical importance of German cockroaches[M]. Rust MK,Owens IM,Reierson DA. Understanding and controlling the German cockroach. New York:Oxford University Press,1995:77-92.
[5] Gore JC,Schal C. Cockroach allergen biology and mitigation in the indoor environment[J]. Ann Rev Entomol,2007,52:439-463. DOI:10.1146/annurev.ento.52.110405.091313.
[6] Kassiri H,Zarrin M,Veys-Behbahani R. Pathogenic fungal species associated with digestive system of Periplaneta americana (Blattaria:Blattidae) trapped from residential dwellings in Ahvaz city,southwestern Iran[J]. J Arthropod Borne Dis,2018,12(1):16-23.
[7] 任东升,吴海霞,郭玉红,等. 2018年全国蜚蠊监测报告[J]. 中国媒介生物学及控制杂志,2019,30(2):142-145. DOI:10.11853/j.issn.1003.8280.2019.02.006.Ren DS,Wu HX,Guo YH,et al. National vectors surveillance report on cockroaches in China,2018[J]. Chin J Vector Biol Control,2019,30(2):142-145. DOI:10.11853/j.issn.1003.8280. 2019.02.006.
[8] 刘峰,刘亚楠,景晓,等. 2010-2015年山东省居民区蟑螂监测分析[J]. 中华卫生杀虫药械,2019,25(2):135-137. DOI:10.19821/j.1671-2781.2019.02.013.Liu F,Liu YN,Jing X,et al. Analysis on monitoring of cockroaches in residential areas in Shandong province from 2010 to 2015[J]. Chin J Hyg Insect Equip,2019,25(2):135-137. DOI:10.19821/j.1671-2781.2019.02.013.
[9] 付学锋,周小洁. 2017年北京市居民家庭蟑螂侵害监测[J]. 首都公共卫生,2019,13(1):45-47. DOI:10.16760/j.cnki.sdggws.2019.01.012.Fu XF,Zhou XJ. Analysis of cockroach infestation rate surveillance in residential family in Beijing in 2017[J]. Cap J Public Health,2019,13(1):45-47. DOI:10.16760/j.cnki.sdggws.2019.01.012.
[10] 余技钢,胡雅劼,李观翠,等. 2014年四川省蟑螂密度监测结果分析[J]. 中华卫生杀虫药械,2016,22(2):153-154,159. DOI:10.19821/j.1671-2781.2016.02.015.Yu JG,Hu YJ,Li GC,et al. Analysis of cockroach surveillance in Sichuan province in 2014[J]. Chin J Hyg Insect Equip,2016,22(2):153-154. DOI:10.19821/j.1671-2781.2016.02.015.
[11] 涂涛田,季恒青,陶晓颖,等. 重庆市德国小蠊对常用卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志,2017,28(3):226-229. DOI:10.11853/j.issn.1003.8280.2017.03.007.Tu TT,Ji HQ,Tao XY,et al. Resistance of Blattella germanica to commonly used insecticides in Chongqing[J]. Chin J Vector Biol Control,2017,28(3):226-229. DOI:10.11853/j.issn.1003. 8280.2017.03.007.
[12] 孙俊,褚宏亮,杨维芳,等. 我国蟑螂抗药性现状与检测方法的研究[J]. 中华卫生杀虫药械,2011,17(1):12-17. DOI:10.19821/j.1671-2781.2011.01.005.Sun J,Chu HL,Yang WF,et al. Current situation and testing method of cockroach resistance in China[J]. Chin J Hyg Insect Equip,2011,17(1):12-17. DOI:10.19821/j.1671-2781.2011. 01.005.
[13] Pan XY,Zhang F. Advances in biological control of the German cockroach,Blattella germanica (L.)[J]. Biol Control,2020,142:104104. DOI:10.1016/j.biocontrol.2019.104104.
[14] Hong MS,Peng GX,Keyhani NO,et al. Application of the entomogenous fungus,Metarhizium anisopliae,for leafroller (Cnaphalocrocis medinalis) control and its effect on rice phyllosphere microbial diversity[J]. Appl Microbiol Biotechnol,2017,101(17):6793-6807. DOI:10.1007/s00253-017-8390-6.