Objective To investigate the mutations of insecticide resistance-related genes through a sequencing analysis of the target genes of insecticides in Anopheles sinensis collected in Guangyuan of Sichuan province, China, and to provide guidance for the chemical control of An. sinensis in this region. Methods A total of 35 female An. sinensis mosquitoes collected in Jianshe village of Longtan township in Guangyuan of Sichuan province in September 2018 were selected, and genomic DNA was extracted after the abdomen was removed. PCR was used to obtain the gene fragments of acetylcholinesterase (ace), voltage-gated sodium channel (VGSC), and gamma-amino butyric acid receptor Rdl subunit (rdl), and then a sequencing analysis was performed. Results In comparison to the gene sequence of reference species, 20.00% of the 35 An. sinensis mosquitoes had resistance mutations, with a frequency of 5.71% for ace resistance allele (119 S), 7.14% and 1.43% for VGSC resistance alleles (1014F and 1014C), and 14.29% for rdl resistance allele (296S). Although there was a low proportion of An. sinensis mosquitoes with resistance mutations (<20.00%), mutations of multiple target genes were observed in more than 70.00% of the mosquitoes with mutation. Conclusion There is a certain proportion of resistance alleles in this An. sinensis population, with a genetic basis of developing high-level resistance, and the presence of mosquitoes with multiple target gene mutations indicates that this An. sinensis population has the possibility of developing into a multidrug-resistant population.
ZHAO Qiong-yao, QIU Xing-hui, YANG Yan, LUO Xing, SONG Xiao-ming, JIA Yong-chao
. Polymorphism and resistance mutation frequency of the target genes of insecticides in Anopheles sinensis in Guangyuan of Sichuan province, China[J]. Chinese Journal of Vector Biology and Control, 2021
, 32(5)
: 541
-545
.
DOI: 10.11853/j.issn.1003.8280.2021.05.006
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