Genetic structure of <i>Anopheles lesteri</i> populations in China based on microsatellite loci

ZHOU Qiu-ming, LIN Lin, DONG Hao-wei, YUAN Hao, BAI Jie, LI Xiang-yu, PENG Heng, MA Ya-jun

doi:10.11853/j.issn.1003.8280.2021.05.004

Chinese Journal of Vector Biology and Control >

2021 , Vol. 32 >Issue 5: 526 - 532

DOI: https://doi.org/10.11853/j.issn.1003.8280.2021.05.004

Malaria Prevention and Control Special Topics

Genetic structure of Anopheles lesteri populations in China based on microsatellite loci

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1. College of Naval Medicine, Naval Medical University, Shanghai 200433, China;
2. Department of Medical Microbiology and Parasitology, College of Basic Medical, Naval Medical University, Shanghai 200433, China

Received date: 2021-03-11

Online published: 2021-10-20

Supported by

Supported by the Municipal Natural Science Foundation of Shanghai of China (No. 19ZR1469600), National Natural Science Foundation of China (No. 31970445) and Major Projects of Logistical Research (No. ALB19J003)

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Abstract

Objective To determine the genetic variation and structure of Anopheles lesteri populations in China using microsatellite loci, and to explore the factors affecting population genetic differentiation. Methods Adult mosquitoes were captured using a sucking tube. An. lesteri was identified based on morphology and rDNA ITS2. Genescan was performed in 9 microsatellite loci. Based on the fragment length polymorphism of microsatellite DNA, genetic diversity within and between populations were calculated. Structure software was used to estimate cluster number and effective population size. Results A total of 216 An. lesteri adults were identified in 17 collection sites, which were pooled into 9 populations for analysis. The mean number of alleles in the populations of An. lesteri ranged from 3.22 to 7.44, the mean expected heterozygosity and the observed heterozygosity were 0.48-0.71 and 0.33-0.47, respectively. The pairwise fixation index ranged from -0.01 to 0.25 (mean, 0.13). The variation within populations (86.55%) was greater than variation between populations (13.45%) that was positively correlated with geographic distance. Structure software showed that An. lesteri populations could be divided into cluster I (populations from Guangdong and Liaoning provinces of China) and cluster II (populations from Hainan, Hubei, Henan, Yunnan, and Sichuan provinces of China, and South Korea). Conclusion The genetic variation of An. lesteri populations in China mainly exists among individuals, with moderate variation levels, and the genetic structure of populations follows the isolation-by-distance model. An. lesteri belongs to two gene pools. Yunnan population was the original gene pool, which gradually migrated to east and north of China and led to the appearance of individuals in the second gene pool.

Key words： Anopheles lesteri; Microsatellite loci; Population genetics; Population structure

Cite this article

ZHOU Qiu-ming, LIN Lin, DONG Hao-wei, YUAN Hao, BAI Jie, LI Xiang-yu, PENG Heng, MA Ya-jun . Genetic structure of Anopheles lesteri populations in China based on microsatellite loci[J]. Chinese Journal of Vector Biology and Control, 2021 , 32(5) : 526 -532 . DOI: 10.11853/j.issn.1003.8280.2021.05.004

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