Transovarial transmission of a new genotype Candidatus Rickettsia longicornii of spotted fever group Rickettsia in Haemaphysalis longicornis

LI Ji-xu, PIAO Wen, JIN Guang-jun

doi:10.11853/j.issn.1003.8280.2021.02.004

Chinese Journal of Vector Biology and Control >

2021 , Vol. 32 >Issue 2: 139 - 143

DOI: https://doi.org/10.11853/j.issn.1003.8280.2021.02.004

Experimental Study

Transovarial transmission of a new genotype Candidatus Rickettsia longicornii of spotted fever group Rickettsia in Haemaphysalis longicornis

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1. Department of Vector Biological Control, Yanbian Korean Autonomous Prefecture Center for Disease Control and Prevention, Yanji, Jilin 133001, China;
2. Hunchun Center for Disease Control and Prevention, Hunchun, Jilin 133300, China

Received date: 2020-02-04

Online published: 2021-04-20

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Abstract

Objective To investigate whether Haemaphysalis longicornis can transovarially transmit the spotted fever group Rickettsia (SFGR) new genotype Candidatus Rickettsia longicornii. Methods Feeding H. longicornis was collected from cattle body surfaces and induced to oviposit in the laboratory. The polymerase chain reaction was used to detect the nucleic acid of Candidatus R. longicornii in tick eggs. The gene sequences of Candidatus R. longicornii in the mothers and eggs of H. longicornis were amplified. An analysis was performed on the homology and phylogenetic relationship. Results A total of 55 feeding H. longicornis (adult female ticks) were collected, among which 21 (38.18%) were positive for Candidatus R. longicornii according to the nucleic acid test. About 2 500 H. longicornis eggs were collected and divided into 50 groups, of which 6 groups were positive for Candidatus R. longicornii. The minimum infection rate was 0.24% in tick eggs. The homology analysis showed that the gene sequences of Candidatus R. longicornii from H. longicornis mothers and eggs had more than 99.79% homology with that of the tick-derived ROK-HL727 strain first found in South Korea. The Candidatus R. longicornii gene sequence homology between H. longicornis mothers and eggs was over 99.69%; the gene sequences were in the same branch and had close phylogenetic relationships with that of the ROK-HL727 strain. Conclusion The infection rate of H. longicornis mothers with the SFGR Candidatus R. longicornii genotype is relatively high, and the genotype can be transovarially transmitted.

Key words： Haemaphysalis longicornis; Tick eggs; SFGR; Candidatus Rickettsia longicornii

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LI Ji-xu, PIAO Wen, JIN Guang-jun . Transovarial transmission of a new genotype Candidatus Rickettsia longicornii of spotted fever group Rickettsia in Haemaphysalis longicornis[J]. Chinese Journal of Vector Biology and Control, 2021 , 32(2) : 139 -143 . DOI: 10.11853/j.issn.1003.8280.2021.02.004

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