Positive rate of overwintering <i>Aedes albopictus</i> eggs and detection of dengue virus carried by the eggs in the former dengue epidemic focus of Ningbo, Zhejiang province, China

MA Min, YANG Si-jia, LI Bao-jun, MA Xiao, GE Bao-xiang, GU Feng, ZHU Meng-yu

doi:10.11853/j.issn.1003.8280.2021.01.012

Chinese Journal of Vector Biology and Control >

2021 , Vol. 32 >Issue 1: 60 - 64

DOI: https://doi.org/10.11853/j.issn.1003.8280.2021.01.012

Vector Surveillance

Positive rate of overwintering Aedes albopictus eggs and detection of dengue virus carried by the eggs in the former dengue epidemic focus of Ningbo, Zhejiang province, China

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1. Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang 315010, China;
2. Haishu District Center for Disease Control and Prevention, Ningbo, Zhejiang 315012, China;
3. Ninghai County Center for Disease Control and Prevention, Ninghai, Zhejiang 315600, China;
4. Beilun District Center for Disease Control and Prevention, Ningbo, Zhejiang 315800, China;
5. Cixi Center for Disease Control and Prevention, Cixi, Zhejiang 315300, China

Received date: 2020-04-28

Online published: 2021-02-20

Supported by

Supported by the Zhejiang Medicine Health Science and Technology Project (No. 2019KY634), Ningbo Medical Science Technology Project (No. 2017A29), Ningbo Health Branding Subject Fund Field Epidemiology (No. PPXK2018-10) and the Social Development Research Project of Haishu District Science and Technology Bureau (No. 201804F028)

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Abstract

Objective To investigate overwintering Aedes albopictus eggs and the dengue virus carried by the eggs in the former dengue epidemic focus in Ningbo, Zhejiang province, China, and to provide scientific evidence for dengue control. Methods During December 2018 to March 2019, standing water and sludge in containers were collected from the former dengue epidemic focus (including the core area, warning area, and monitoring area) of Haishu district and three non-epidemic areas (Beilun district, Cixi city, and Ninghai county) in Ningbo, for incubation of Ae. albopictus eggs under laboratory conditions. Real-time RT-PCR was used to detect the dengue virus carried by the eggs. The chi-square test and logistic regression analysis were used to determine the influencing factors of the positive rate of overwintering Ae. albopictus eggs. Results A total of 884 samples were collected, and the positive rate of overwintering Ae. albopictus eggs was 11.99%. There were significant differences in the positive rate of overwintering Ae. albopictus eggs between different dengue control areas (χ²=31.227, P<0.001) and between different water containers (χ²=38.044, P<0.001). According to multivariate analysis, the positive rates for the warning area, monitoring area, and non-epidemic area were 5.33 (95% confidence interval[CI]:1.16-24.49), 6.26 (95%CI:1.37-28.65), and 11.56 (95%CI:2.75-48.50) times that for the core area, respectively. The positive rates for waste tires and vegetative containers were 3.59 (95%CI:1.79-7.20) and 3.77 (95%CI:1.82-7.79) times that for stationary containers, respectively. All test results for the nucleic acid of dengue virus were negative. Conclusion The warning area and monitoring area of the former dengue epidemic focus, non-epidemic areas, waste tires, and vegetative containers are the main breeding sites for overwintering Ae. albopictus eggs. It is suggested that relevant departments should take timely measures focusing on the removal of standing water to control mosquitoes in early spring.

Key words： Aedes albopictus; Overwintering egg; Influencing factor; Dengue virus; Real-time RT-PCR

Cite this article

MA Min, YANG Si-jia, LI Bao-jun, MA Xiao, GE Bao-xiang, GU Feng, ZHU Meng-yu . Positive rate of overwintering Aedes albopictus eggs and detection of dengue virus carried by the eggs in the former dengue epidemic focus of Ningbo, Zhejiang province, China[J]. Chinese Journal of Vector Biology and Control, 2021 , 32(1) : 60 -64 . DOI: 10.11853/j.issn.1003.8280.2021.01.012

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