ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
A transcriptome study of Blattella germanica in response to low-temperature stress
Received date: 2020-05-28
Online published: 2021-02-20
Objective To investigate the transcriptome of Blattella germanica in response to low-temperature stress, the functional enrichment and metabolic pathways of low-temperature response genes, and the molecular mechanism of low-temperature response in B. germanica, and to improve the understanding of the molecular mechanism of temperature adaptation in B. germanica. Methods B. germanica adults were fed in this laboratory; B. germanica adults in the 25℃ were selected as controls, and transcriptome sequencing was performed for those placed in the 4℃ for 2 hours of low-temperature treatment to identify differentially expressed genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to analyze the functional enrichment and metabolic pathways of genes. Results The sequencing data obtained 44 589 020 non-redundant transcripts, and calculation showed that 332 genes were upregulated and 371 genes were downregulated in response to low temperature. The low-temperature response genes were enriched into 27 GO terms and 35 KEGG metabolic pathways, including carboxylic acid metabolism, stress response, carbohydrate metabolism, neurotransmitter secretion, carbonate dehydratase activity, oxidoreductase activity, lyase activity, pyruvate dehydrogenase activity, pyruvate dehydrogenase complex, water transport, abiotic stress response, channel activity, multicellular tissue process, muscle membrane, plasma membrane, steroid hormone response, organic matter response, exopeptidase, protein metabolism, "P-P hydrolysis" kinesin transmembrane transporter activity, ER-targeted protein, hydrolase activity, nutrition storage activity, transferase activity, metal carboxypeptidase activity, lipid transporter activity, and cell division. Conclusion B. germanica may respond to low-temperature stress from 27 aspects including carboxylic acid metabolism and stress response, which provides a basis for clarifying the molecular mechanisms of response and tolerance to low temperature in B. germanica.
Key words： Blattella germanica; Low temperature; Gene expression
ZHANG Ya-ming, CHEN Hong-yu, ZHANG Wen-jia, WANG Yue, YU Yang, CHEN Qu . A transcriptome study of Blattella germanica in response to low-temperature stress[J]. Chinese Journal of Vector Biology and Control, 2021 , 32(1) : 34 -37 . DOI: 10.11853/j.issn.1003.8280.2021.01.006
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