Objective To investigate the mosquitoes and arboviruses causing the Japanese encephalitis epidemic in Tumoteyou banner in Baotou of Inner Mongolia autonomous region, China in 2018. Methods The lamp trapping method was used to collect mosquito specimens in Tumoteyou banner of Baotou, and the mosquito specimens were classified and numbered based on morphological identification and were then stored in liquid nitrogen. Reverse transcription-polymerase chain reaction was used to identify mosquito viruses, and several bioinformatics softwares, including DNAStar, were used to analyze the molecular biological features of the nucleotide sequence of the virus. Results On September 8-12, 2018, a total of 208 139 mosquitoes belonging to 5 species were collected in Tumoteyou banner of Baotou, and Aedes dorsalis, Culex pipiens pallens, Cx. tritaeniorhynchus, Cx. modestus, and Ae. flavescens accounted for 46.56%, 35.77%, 8.12%, 5.25%, and 4.30%, respectively, of the mosquitoes collected. A total of 198 mosquito specimens were ground, 2 of which tested positive for flavivirus, with 1 positive for Japanese encephalitis virus (JEV) and 1 positive for Tahyna virus. Complete genome sequencing was performed for the JEV-positive specimen, and a complete genome sequence of 10 880 bp was obtained. Genotyping was performed using the PrM segment of the virus (456-695 nucleotides of the genome), and the sequence of JEV was determined as genotype I. Compared with the strain SA14-14-2 for live attenuated vaccine against Japanese encephalitis in China, the virus has a nucleotide difference of 12.59% and an amino acid difference of 2.96%. Conclusion The complete genome sequence of genotype I JEV is obtained for the first time in Ae. dorsalis in Inner Mongolia autonomous region.
NAN Xiao-wei, XIE Xin-xia, YU Hong-min, SI Xiao-yan, CHEN Ji-lai, ZHANG Chen-guang, ZHANG Xiu-hong
. An investigation of the vector mosquitoes and arboviruses during a Japanese encephalitis epidemic in Baotou of Inner Mongolia autonomous region, China, 2018[J]. Chinese Journal of Vector Biology and Control, 2020
, 31(6)
: 652
-656
.
DOI: 10.11853/j.issn.1003.8280.2020.06.005
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