Objective To investigate the molecular epidemiological characteristics of human brucellosis in Alxa league, Inner Mongolia autonomous region (Inner Mongolia), China. Methods The clinical isolates of suspected Brucella with a positive blood culture result during 2015 to 2019 were identified by conventional biological methods and multiplex PCR assay. The molecular epidemiological characteristics of Brucella were investigated using multiple-locus variable-number tandem repeat analysis (MLVA). Results Twenty-three suspected Brucella strains were isolated from the confirmed cases of brucellosis in Alxa league from 2015 to 2019, and were identified as B. melitensis biovar 3 using conventional biological methods and multiplex PCR assay. Among the 23 cases, herdsmen accounted for 30.43% (7/23) and farmers accounted for 39.13% (9/23), both of whom had a history of sheep contact. The positive rate of Brucella antibody in serum was 78.26% (18/23). The MLVA showed that the 23 strains had genotypes of the main epidemic B. melitensis strains in China. According to the three loci with differences, the strains isolated from Bayanmuren Sumu belonged mainly to one group, and the two strains from Jilantai town belonged to another group. The 23 strains were associated with the isolates from Ningxia, Liaoning, Shandong, Zhejiang, Shanghai, and Guangdong (provinces, autonomous regions, and municipalities directly under the central government), and were not associated with isolates from its neighboring province Gansu. Conclusion The Brucella strains from Alxa league, Inner Mongolia are consistent with the epidemic strains of brucellosis in China, and are associated with the isolates from Ningxia, Liaoning, Shandong, Zhejiang, Shanghai, and Guangdong (provinces, autonomous regions, and municipalities directly under the central government).
XU Chuang-ze, PIAO Dong-ri, JIANG Hai, LIU Hui-lan, LI Meng, TIAN Hai-rong, JIAO Hong-yan, TIAN Guo-zhong
. Study on epidemiological characteristics and pathogen typing of brucellosis in Alxa league, Inner Mongolia, China[J]. Chinese Journal of Vector Biology and Control, 2020
, 31(6)
: 648
-651
.
DOI: 10.11853/j.issn.1003.8280.2020.06.004
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