Study on the diversity of intestinal microbiota of wild rodents from the central mountainous area of Hainan Island, China

ZHOU Jie, ZHOU Xiao-jun, XUAN Duan-duan, PAN Liang-yuan, LIANG Jia-xin, CHEN Zong-liang, ZHANG Meng-meng, SU Rong-yu, BIAN Chen-bin, DONG Yu-xin, HU Shou-kui, NIU Li-na

doi:10.11853/j.issn.1003.8280.2020.05.019

Chinese Journal of Vector Biology and Control >

2020 , Vol. 31 >Issue 5: 596 - 601

DOI: https://doi.org/10.11853/j.issn.1003.8280.2020.05.019

Investigation

Study on the diversity of intestinal microbiota of wild rodents from the central mountainous area of Hainan Island, China

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1 Hainan Medical College, Haikou 571199, Hainan Province, China;
2 Peking University Shougang Hospital

Received date: 2020-04-23

Online published: 2020-10-20

Supported by

Supported by the Project Funding of Education Department of Hainan Province in 2019 (No. Hnky2019ZD-27), National Natural Science Foundation of China (No. 81760376), National Innovation and Entrepreneurship Training Program for College Students in 2018 (No. 20181181024), Innovative Experimental Project of Hainan Medical College Students in 2018 (No. HYCX2018024) and Innovation and Entrepreneurship Training Program for College Students in Hainan Province in 2019 (No. S201911810034)

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Abstract

Objective To investigate the diversity of intestinal microbiota of wild rodents from the central mountainous area of Hainan Island, China. Methods The high-throughput sequencing technology was used to sequence the DNA extracted from fecal samples of wild rodents, and the obtained sequence data were analyzed using bioinformatic methods to investigate the composition and diversity of intestinal microbiota. Results A total of 1 569 543 valid sequences were obtained from the fecal samples of 20 wild rodents. The number of operational taxonomic units ranged from 540 to 1 274. At the phylum level, the main flora of the studied wild rodents was Firmicutes (55.83%), followed by Bacteroidetes (27.74%). At the genus level, the top 10 bacterial genera were Helicobacter, Lactobacillus, Clostridium, Escherichia-Shigella, Streptococcus, Lachnospiraceae, Faecalitalea, Fusobacterium, Mycoplasma, and Romboutsia. Conclusion High-throughput sequencing analysis revealed that the structure of the intestinal microbiota of wild rodents in the central mountainous area of Hainan Island was complex, with abundant species and large differences among individuals. Helicobacter had the highest relative abundance in six fecal samples, which was of significance and value for further investigations.

Key words： Wild rodents; High-throughput sequencing; Intestinal microbiota diversity

Cite this article

ZHOU Jie, ZHOU Xiao-jun, XUAN Duan-duan, PAN Liang-yuan, LIANG Jia-xin, CHEN Zong-liang, ZHANG Meng-meng, SU Rong-yu, BIAN Chen-bin, DONG Yu-xin, HU Shou-kui, NIU Li-na . Study on the diversity of intestinal microbiota of wild rodents from the central mountainous area of Hainan Island, China[J]. Chinese Journal of Vector Biology and Control, 2020 , 31(5) : 596 -601 . DOI: 10.11853/j.issn.1003.8280.2020.05.019

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