Objective To investigate the fauna and distribution of fleas in the southeast of Tibet autonomous region, and to provide a basis for further research on flea fauna in the region. Methods Linzhi in the southeast of Tibet was taken as the area for research, and the small mammals and their parasitic fleas in the farming areas of different altitudes were taken as the study subjects; the small mammals were captured using large-size mouse traps and their parasitic fleas were investigated and sampled, and the obtained specimens were classified and identified in the laboratory. Results From the specimens of the genus Genoneopsylla Wu, Wu et Liu, 1966, female specimens of G.zhiyini Wu, Ge et Lan, 2003 were identified for the first time. The morphology and characteristics of the female flea species were described in this article. Conclusion Up to now, four species of the genus Genoneopsylla distributed in Tibet have been identified. With increasing investigation and taxonomic study of the fleas, the number of species of the genus will become larger.