Objective To determine the nucleic acid sequence of the dengue type 1 virus (DENV-1) detected in the local dengue outbreak in Quzhou, Zhejiang province, China, 2019, and to analyze its molecular characteristics. Methods Serum samples were collected from dengue patients; colloidal gold immunochromatographic assay and quantitative real-time PCR (RT-qPCR) were used to detect the NS1 antigen and nucleic acid of dengue virus, respectively. The RT-qPCR assay was used to amplify the envelope gene of dengue virus, which was then sequenced. The genotype of the isolated strains was analyzed, and the homology and phylogenetic analyses were performed with the dengue strains isolated from other countries and regions. Results The serum samples of patients were positive for the NS1 antigen and nucleic acid of dengue virus. The gene sequence alignment and phylogenetic analysis showed that 8 dengue virus strains all belonged to DENV-1 subgenotype GI, which had the closest phylogenetic relationship with the strain JF960228 (Singapore, 2010), with a mutation at virulence locus 1. Conclusion It was confirmed by etiology, serology, and molecular biology that the local dengue outbreak was caused by DENV-1, which may originate from Southeast Asia. It is necessary to strengthen the prevention and control of cross-border spread of dengue fever in China and this region.
YANG Rui-jun, HUANG Shi-teng, LYU Lei, CAO Guo-ping, YOU Jia-ling, WAN Sheng
. Molecular epidemiological study of local dengue outbreak in Quzhou, Zhejiang province, China, 2019[J]. Chinese Journal of Vector Biology and Control, 2020
, 31(5)
: 521
-525
.
DOI: 10.11853/j.issn.1003.8280.2020.05.004
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