Genetic characteristics of <i>Rattus tanezumi</i> populations at four ports of entry in southwest Yunnan province, China

LIU Ying-ying, CAO Xiao-mei, CI Ying, YANG Yan, LU Yun-lan, ZHANG Xiao-long, FANG Zhi-qiang

doi:10.11853/j.issn.1003.8280.2020.04.015

Chinese Journal of Vector Biology and Control >

2020 , Vol. 31 >Issue 4: 451 - 456

DOI: https://doi.org/10.11853/j.issn.1003.8280.2020.04.015

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Genetic characteristics of Rattus tanezumi populations at four ports of entry in southwest Yunnan province, China

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1 Chinese Academy of Inspection and Quarantine Institute of Health Inspection and Quarantine, Beijing 100176, China;
2 Yunnan International Travel Healthcare Center

Received date: 2020-02-10

Online published: 2020-08-20

Supported by

Supported by the National Science and Technology Major Project of China (No. 2017ZX10303404)

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Abstract

Objective To investigate the genetic diversity and phylogenetic relationship of Rattus tanezumi populations at four ports of entry (POEs) (Jinshuihe, Mohan, Qingshuihe, and Jiegao) in Yunnan province, China. Methods Fifty-five R. tanezumi rats captured at the four POEs from 2014 to 2015 were dissected, and their liver tissues were taken for genomic DNA extraction. PCR was used to analyze the mitochondrial DNA cytochrome b (Cytb) gene, and the bioinformatics software Clustal X 2.0 and MEGA 7.0 were applied to analyze the Cytb gene sequence. The nucleotide composition and codon usage frequency of the Cytb gene sequence were calculated. The genetic distance was estimated, and the phylogenetic tree was constructed by the neighbor-joining (NJ) method. Results The base composition A+T and C+G of the Cytb gene sequence accounted for about 59.77% and 40.23%, respectively. The usage frequencies of the codons UCA, CUA, and CCA were 3.19%, 3.11%, and 3.09%, respectively. The genetic distance of the Cytb gene of R. tanezumi between Jiegao and Qingshuihe POEs was 0.000, and that between Jinshuihe and Qingshuihe or Jiegao POEs was 0.008. With Mus musculus and R. norvegicus as outgroups, the NJ phylogenetic tree showed that the rats from Qingshuihe port first gathered with those from Jiegao on a branch, then with those from Mohan port, and finally with those from Jinshuihe port. Conclusion The content of A+T in the Cytb gene sequence is significantly higher than that of C+G, suggesting a potential A+T bias in the Cytb gene of R. tanezumi. Genetic distance and NJ phylogenetic tree analysis show that R. tanezumi from Qingshuihe port has the closest relationship with that from Jiegao port, and the second closest relationship with that from Mohan. Rattus tanezumi from Jinshuihe has a relatively far genetic relationship with that from the other three POEs.

Key words： Ports of entry in Yunnan province; Rattus tanezumi; Cytochrome b gene

Cite this article

LIU Ying-ying, CAO Xiao-mei, CI Ying, YANG Yan, LU Yun-lan, ZHANG Xiao-long, FANG Zhi-qiang . Genetic characteristics of Rattus tanezumi populations at four ports of entry in southwest Yunnan province, China[J]. Chinese Journal of Vector Biology and Control, 2020 , 31(4) : 451 -456 . DOI: 10.11853/j.issn.1003.8280.2020.04.015

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