Objective The olfactory system plays an important role in mosquito behaviors such as blood sucking and oviposition site selection. This study compared the expression level of odorant receptor 10 gene (OR10) in Aedes albopictus before and after blood sucking. Methods Reverse transcription-polymerase chain reaction (RT-PCR) was performed to amplify the OR10 gene, and quantitative real-time PCR was used to determine the expression levels of OR10 gene in male Ae. albopictus, and female Ae. albopictus before and after blood sucking. Results The open reading frame of the OR10 gene in Ae. albopictus obtained by amplification was 1 128 bp in length, encoding 376 amino acids. The amino acid sequence had an identity of 86.13% and 69.69%, respectively, with that in Ae. aegypti and Anopheles gambiae. The results of quantitative real-time PCR showed that male Ae. albopictus had the lowest expression level of OR10 gene of 1; female Ae. albopictus had a higher expression level (1.535±0.274) than male Ae. albopictus; female Ae. albopictus had the highest expression level of OR10 gene (2.284±0.203) after blood sucking; the expression level of OR10 gene in female Ae. albopictus changed significantly before and after blood sucking (F=32.067, P=0.001). Conclusion The expression level of OR10 in Ae. albopictus changes significantly before and after blood sucking, which further proves that OR10 may be involved in the mosquito behavior of oviposition site selection after blood sucking.
HUANG Xiao-dan, YANG Lin-lin, LIU Lu-hong, CHENG Peng, LIU Hong-mei
. Expression level of olfactory receptor 10 gene in Aedes albopictus before and after blood sucking[J]. Chinese Journal of Vector Biology and Control, 2020
, 31(3)
: 331
-334
.
DOI: 10.11853/j.issn.1003.8280.2020.03.017
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