Objective To investigate the effects of intestinal bacteria in Musca domestica larvae on their feeding and oviposition into the adulthood. Methods Traditional methods were used for isolation and culture of culturable non-anaerobic bacteria from the intestinal tracts of 3-day-old M. domestica larvae. These bacteria were used to carry out feeding and oviposition preference experiments on M. domestica adults. With SPSS 20.0 software for statistical analysis, one-way analysis of variance was conducted for comparison between groups, and the t-test was used for comparison between two independent samples. Results Ten bacteria belonging to 9 genera were isolated from the intestinal tracts of M. domestica larvae:Enterobacter hormaechei, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter bereziniae, Providencia stuartii, E. cloacae, Lactococcus lactis, Lysinibacillus fusiformis, P. vermicola, and Bacillus safensis. E. hormaechei, L. lactis, and E. cloacae were found to exert attracting effects on feeding of M. domestica adults, yielding attracting rates of (80.88±5.60)%, (60.77±4.13)%, and (61.59±4.13)%, respectively, and the other seven bacteria were found to inhibit feeding. Ten bacteria were all revealed to have attracting effects on oviposition of M. domestica adults. E. hormaechei, E. cloacae, L. lactis, L. fusiformis, and B. safensis showed strongest attracting effects, with attracting rates of 100%. Conclusion It has been confirmed that intestinal bacteria in M. domestica larvae have effects on their feeding and oviposition into their adulthood, and it is clarified that M. domestica adults' feeding and oviposition are mainly regulated by nutrition and odor, respectively. The results lay a foundation for research and development of new bacterial repellents or attractants of M. domestica adults.
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