Investigation of <i>Rickettsia typhi</i> in small mammals in Luxi county, Yunnan province, China

YUAN Qing-hong, YANG Xiang-dong

doi:10.11853/j.issn.1003.8280.2020.03.009

Chinese Journal of Vector Biology and Control >

2020 , Vol. 31 >Issue 3: 289 - 293

DOI: https://doi.org/10.11853/j.issn.1003.8280.2020.03.009

Original Reports

Investigation of Rickettsia typhi in small mammals in Luxi county, Yunnan province, China

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Yunnan Provincial Key Laboratory of Natural Focal Disease Control and Prevention, Yunnan Institute of Endemic Disease Control and Prevention, Dali 671000, Yunnan Province, China

Received date: 2020-01-21

Online published: 2020-06-20

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Supported by the Xu Jian-guo Academician Work Station (No. 2018IC155)

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Abstract

Objective To investigate the general status and molecular biological characteristics of Rickettsia typhi carried by small mammals in Luxi county, Yunnan province, China, as well as the national foci of typhus, and to provide a scientific basis for prevention and control of Rickettsia diseases. Methods Rat cages and rat traps were used to capture smalls in residential and wild areas in Luxi county. The captured animals were classified before their blood was collected from them alive for their sera. Indirect immunofluorescence assay was adopted to detect R. typhi antibodies in their sera. Spleens were cut off for DNA extraction and nested polymerase chain reaction (PCR) analysis to detect the groEL gene of R. typhi. Positive specimens were sequenced and analyzed by the Basic Local Alignment Search Tool (BLAST) provided by the National Center for Biotechnology Information (NCBI), and their phylogenetic trees were constructed for homology and evolution analysis. Results A total of 193 small mammals were captured in Luxi county, 64 of which were in residential areas with Rattus tanezumi as the dominant species and 129 of which were in wild areas with Eothenomys miletus and Apodemus draco as the dominant species. Eighty-three sera samples were obtained. Nested PCR analysis of their spleens found all specimens in residential areas negative and 2 specimens (LX2, LX89) in wild areas positive for the groEL gene. The positive specimens were from E. miletus, yielding a carrying rate of 1.04%. The two positive specimens were sequenced and aligned by BLAST with known sequences in GenBank. LX2 and LX89 showed 100% identity, and they displayed 100% and 98% identity with R. mooseri (GenBank AF075440), respectively. Their phylogenetic trees suggested that they were in the same branch and closely related to AF075440, AY191590,and AY191591 strains of R. mooseri. Four of eighty-three rodents' sera were positive for R. mooseri antibodies with a positive rate of 4.82%. Conclusion There are natural foci of typhus mainly hosted by E. miletus in Luxi county, Yunnan province, China.

Key words： Small mammals; Rickettsia; Typhus; Rickettsia mooseri

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YUAN Qing-hong, YANG Xiang-dong . Investigation of Rickettsia typhi in small mammals in Luxi county, Yunnan province, China[J]. Chinese Journal of Vector Biology and Control, 2020 , 31(3) : 289 -293 . DOI: 10.11853/j.issn.1003.8280.2020.03.009

References

[1] 张丽娟. 中国立克次体病监测及防治现状与展望[J]. 疾病监测,2007,22(9):577-579. DOI:10.3784/j.issn.1003-9961. 2007.09.001. Zhang LJ. Prospect and status of rickettsioses surveillance in China[J]. Dis Surveill,2007,22(9):577-579. DOI:10.3784/j.issn.1003-9961.2007.09.001.
[2] 张丽娟,付秀萍,贺金荣. 我国近十年斑疹伤寒疫情概况及分析[J]. 中国预防医学杂志,2005,6(5):415-418. Zhang LJ,Fu XP,He JR. Analysis on the epidemic characteristics of typhus from 1994-2003 in China[J]. China Prev Med,2005,6(5):415-418.
[3] 柴程良,孙继民,陆群英,等. 浙江省鼠形动物蜱源立克次体感染状况调查[J]. 浙江预防医学,2014,26(7):659-663. Chai CL,Sun JM,Lu QY,et al. A cross-sectional study on tick-borne Rickettsiae infections among murine-like animals[J]. Zhejiang Prev Med,2014,26(7):659-663.
[4] 亚红祥,张云智,习严梅. 云南省2005-2014年斑疹伤寒流行特征分析[J]. 中国媒介生物学及控制杂志,2017,28(4):359-361,378. DOI:10.11853/j.issn.1003.8280.2017.04.013. Ya HX,Zhang YZ,Xi YM. Analysis on epidemiological characteristics of typhus in Yunnan province,China in 2005-2014[J]. Chin J Vector Biol Control,2017,28(4):359-361,378. DOI:10.11853/j.issn.1003.8280.2017.04.013.
[5] 郑维斌,李加全,杨妮,等. 保山市2009年一起地方性斑疹伤寒流行的调查分析[J]. 海峡预防医学杂志,2010,16(5):36-37. Zheng WB,Li JQ,Yang N,et al. Investigation and analysis of a local typhus epidemic in Baoshan city in 2009[J]. Channel Prev Med,2010,16(5):36-37.
[6] 王庆新,李育民,刘继政. 某村地方性斑疹伤寒暴发的调查[J]. 现代预防医学,2006,33(6):1010. DOI:10.3969/j.issn.1003-8507.2006.06.075. Wang QX,Li YM,Liu JZ. Investigation on the outbreak of local typhus in a village[J]. Mod Prev Med,2006,3(6):1010. DOI:10.3969/j.issn.1003-8507.2006.06.075.
[7] 张海林,苏梅惠,姚娜,等. 云南省西双版纳州鼠型斑疹伤寒暴发的调查[J]. 中国人兽共患病学报,2014,30(12):1272-1280. DOI:10.3969/cjz.j.issn.1002-2694.2014.12.021. Zhang HL,Su MH,Yao N,et al. Murine typhus in Xishuangbanna prefecture,Yunnan province,China[J]. Chin J Zoonoses,2014,30(12):1272-1280. DOI:10.3969/cjz.j.issn.1002-2694.2014.12.021.
[8] 亚红祥,张海林,周济华,等. 云南省保山市一起地方性斑疹伤寒暴发的调查[J]. 中华流行病学杂志,2011,32(1):47-50. DOI:10.3760/cma.j.issn.0254-6450.2011.01.012. Ya HX,Zhang HL,Zhou JH,et al. An outbreak of endemic typhus in Baoshan city,Yunnan province[J]. Chin J Epidemiol,2011,32(1):47-50. DOI:10.3760/cma.j.issn.0254-6450. 2011.01.012.
[9] 袁庆虹,杨卫红,米竹青,等. 云南大理地区发热病人恙虫病抗体调查[J]. 地方病通报,2001,16(2):31-32. DOI:10.3969/j.issn.1000-3711.2001.02.011. Yuan QH,Yang WH,Mi ZQ,et al. Investigation on antibody against tsutsugamushi disease in febrile patients in Dali Yunnan province[J]. Endem Dis Bull,2001,16(2):31-32. DOI:10.3969/j.issn.1000-3711.2001.02.011.
[10] 王园园,梁长威,赫兢,等. 斑疹伤寒群立克次体groEL基因荧光定量聚合酶链反应方法的建立及临床病例检测分析[J]. 疾病监测,2011,26(1):8-11. DOI:10.3784/j.issn.1003-9961. 2011.01.004. Wang YY,Liang CW,He J,et al. Establishment of real-time PCR assay for typhus group rickettsia groEL genes and clinical case detection[J]. Dis Surveill,2011,26(1):8-11. DOI:10.3784/j.issn.1003-9961.2011.01.004.
[11] 吴捷,王少玲,马炎,等. 海南省2009-2011年发热病人立克次体病检测及结果分析[J]. 中国卫生检验杂志,2015,25(9):1311-1313,1316. Wu J,Wang SL,Ma Y,et al. Analysis of the detection results of rickettsia disease among patients with fever in Hainan province from 2009 to 2011[J]. Chin J Health Lab Tec,2015,25(9):1311-1313,1316.
[12] Rakotonanahary RJL,Harrison A,Maina AN,et al. Molecular and serological evidence of flea-associated typhus group and spotted fever group rickettsial infections in Madagascar[J]. Parasit Vectors,2017,10(1):125. DOI:10.1186/s13071-017-2061-4.
[13] 张丽娟,付秀萍,范明远. 我国立克次体研究与立克次体病的流行现状[J]. 热带病与寄生虫学,2005,3(1):37-42. DOI:10.3969/j.issn.1672-2302.2005.01.016. Zhang LJ,Fu XP,Fan MY. Study on rickettsia and epidemic situation of rickettsia in China[J]. J Trop Dis Parasitol,2005,3(1):37-42. DOI:10.3969/j.issn.1672-2302.2005.01.016.
[14] 林永丽,李旭霞,方旭东. 美国立克次体病的研究现状[J]. 海军医学杂志,2014,35(1):75-76. DOI:10.3969/j.issn.1009-0754.2014.01.031. Lin YL,Li XX,Fang XD. Research status of rickettsia in the United States[J]. J Navy Med,2014,35(1):75-76. DOI:10.3969/j.issn.1009-0754.2014.01.031.
[15] Gérardin P,Zemali N,Bactora M,et al. Seroprevalence of typhus group and spotted fever group rickettsia exposures on Reunion island[J]. BMC Res Notes,2019,12(1):387. DOI:10.1186/s13104-019-4416-8.
[16] Teoh YT,Hii SF,Stevenson MA,et al. Serological evidence of exposure to Rickettsia felis and Rickettsia typhi in Australian veterinarians[J]. Parasit Vectors,2017,10(1):129. DOI:10.1186/s13071-017-2075-y.
[17] Theunissen C,Cnops L,Van Esbroeck M,et al. Acute-phase diagnosis of murine and scrub typhus in Belgian travelers by polymerase chain reaction:a case report[J]. BMC Infect Dis,2017,17(1):273. DOI:10.1186/s12879-017-2385-x.
[18] Tsioutis C,Zafeiri M,Avramopoulos A,et al. Clinical and laboratory characteristics,epidemiology,and outcomes of murine typhus:a systematic review[J]. Acta Trop,2017,166:17-24. DOI:10.1016/j.actatropica.2016.10.018.
[19] Helgren TR,Chen CL,Wangtrakuldee P,et al. Rickettsia prowazekii methionine aminopeptidase as a promising target for the development of antibacterial agents[J]. Bioorgan Med Chem,2017,25(3):813-824. DOI:10.1016/j.bmc.2016.11.013.
[20] Xu ZQ,Zhu XC,Lu QY,et al. Misdiagnosed murine typhus in a patient with multiple cerebral infarctions and hemorrhage:a case report[J]. BMC Neurol,2015,15(1):121. DOI:10.1186/s12883-015-0383-4.
[21] 孙印旗,董妥,姜霞,等. 2009-2012年河北鼠型斑疹伤寒流行概况及实验室调查分析[J]. 中国人兽共患病学报,2015,31(6):552-555. DOI:10.3969/cjz.j.issn.1002-2694.2015.06. 012. Sun YQ,Dong T,Jiang X,et al. Epidemiological characterizes and laboratory investigation of endemic typhus in Hebei province,2009-2012[J]. Chin J Zoonoses,2015,31(6):552-555. DOI:10.3969/cjz.j.issn.1002-2694.2015.06.012.
[22] 徐琪毅,李宏英,李飞,等. 新疆伊犁州农村儿童媒介传播立克次体病血清流行病学调查[J]. 中国媒介生物学及控制杂志,2016,27(1):58-60. DOI:10.11853/j.issn.1003.4692. 2016.01.019. Xu QY,Li HY,Li F,et al. Serological investigation of vector born rickettsioses in agrarian children in Yili prefecture,Xinjiang Uygur autonomous region[J]. Chin J Vector Biol Control,2016,27(1):58-60. DOI:10.11853/j.issn.1003.4692. 2016.01.019.
[23] 常利涛,刀志宏,梁长威,等. 云南省人和家畜立克次体病血清流行病学调查[J]. 中国人兽共患病学报,2010,26(2):189-192. DOI:10.3969/j.issn.1002-2694.2010.02.024. Chang LT,Dao ZH,Liang CW,et al. Sero-epidemiologic investigation on rickettsiosis of humans and domestic animals in Yunnan province[J]. Chin J Zoonoses,2010,26(2):189-192. DOI:10.3969/j.issn.1002-2694.2010.02.024.
[24] 李加全,杨和仙. 云南省保山市2005-2013年斑疹伤寒流行特征与影响因素分析[J]. 医学动物防制,2015,31(3):277-279,282. DOI:10.7629/yxdwfz201503013. Li JQ,Yang HX. Typhus epidemic characteristics and influence factor analysis of Baoshan city in Yunnan province during 2005-2013[J]. J Med Pest Control,2015,31(3):277-279,282. DOI:10.7629/yxdwfz201503013.
[25] 高丽芬,吴学林,胡海梅,等. 2006-2017年楚雄州3种鼠传疾病流行分析[J]. 现代预防医学,2019,46(9):1551-1554,1562. Gao LF,Wu XL,Hu HM,et al. Epidemiological analysis of three rodent-borne diseases in Chuxiong,2006-2017[J]. Mod Prev Med,2019,46(9):1551-1554,1562.
[26] 李园园. 西双版纳州勐海县2004-2010年斑疹伤寒流行特征分析[J]. 中国媒介生物学及控制杂志,2012,23(2):179. Li YY. Analysis of the epidemiological characteristics of typhus from 2004 to 2010 in Menghai county,Xishuangbanna[J]. Chin J Vector Biol Control,2012,23(2):179.
[27] Zhang LJ,Li XM,Zhang DR,et al. Molecular epidemic survey on co-prevalence of scrub typhus and marine typhus in Yuxi city,Yunnan province of China[J]. Chin Med J,2007,120(15):1314-1318. DOI:10.1097/00029330-200708010-00004.
[28] 章域震,王勇,杨卫红,等. 云南省曲靖市啮齿动物的立克次体血清学和分子流行病学调查[J]. 中国人兽共患病学报,2015,31(1):35-40. DOI:10.3969/cjz.j.issn.1002-2694.2015. 01.008. Zhang YZ,Wang Y,Yang WH,et al. Seroprevalence and molecular biological investigation of commonly known rickettsia species in rodents in Qujing city,Yunnan province,China[J]. Chin J Zoonoses,2015,31(1):35-40. DOI:10.3969/cjz.j.issn.1002-2694.2015.01.008.

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