Objective To identify a newly recorded species discovered in tick surveillance in Zhejiang province, China, 2018. Methods Ticks were collected from grasses, shrubs, and the body surface of domestic animals in Jiaojiang district, Anji county, and Pujiang county of Zhejiang province, China. Morphological identification was conducted, followed by amplification of the mitochondrial 16S rRNA and cytochrome coxidase I (COⅠ) genes by PCR. After sequencing, homology analyses were performed. Results In 2018, 13 male ticks, a newly recorded species captured in Zhejiang province, were morphologically identified as Haemaphysalis flava Neumann (1897). The mitochondrial 16S rRNA gene of this tick displayed 100% identity to the known fragment of that gene of H. flava (Henan, KX450282.1); in comparison with other Haemaphysalis, the homology was 86.9%-92.2% and the divergence was 8.2%-14.7%. The mitochondrial COⅠgene of this tick showed 99.8% identity to the known COⅠ gene fragment of H. flava (Hunan, KJ195464.1); in comparison with other Haemaphysalis, the homology was 84.7%-90.7% and the divergence was 10.0%-17.2%. Conclusion The combination of morphological and molecular biological identification methods can accurately identify the species of ticks.
LIU Ying, HOU Juan, LI Tian-qi, GUAN Chang-hui, WU Xue, WU Hong-zhao, LING Feng, ZHANG Rong, SHI Xu-guang, REN Jiang-ping, CHEN En-fu, SUN Ji-min
. Morphological and molecular identification of one newly recorded species of Ixodidae in Zhejiang province, China[J]. Chinese Journal of Vector Biology and Control, 2020
, 31(2)
: 164
-168
.
DOI: 10.11853/j.issn.1003.8280.2020.02.009
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