Objective To investigate the genotypes and distribution characteristics of the ace gene of the dengue vector Aedes albopictus (field population) in different regions of China, and to preliminarily explore their resistance mechanism to organophosphorus and carbamate insecticides. Methods A total of 422 larvae, pupae, or adults of Ae. albopictus were collected at 14 sampling sites (14 geographical populations) in Haikou of Hainan province, Guangzhou of Guangdong province, Jinghong of Yunnan province, and Xingyi of Guizhou province from June to September 2018, and they were identified by morphology or molecular biology, soaked in anhydrous ethanol, and stored at -20℃. DNA was extracted from individual mosquito, and the partial fragment of the ace gene was amplified by PCR, sequenced, and analyzed for mutation at G119. Results There were only a few base mutations but no amino acid mutations at G119 of the ace gene. Two alleles were detected, namely wild-type GGA/G (832/98.58%) and mutant-type GGG/G (12/1.42%), and one genotype was detected, namely wild-type homozygote G/G (422/100%). Conclusion Only a few individuals of Ae. albopictus showed a base mutation at G119 of the ace gene, but the base substitution did not cause the corresponding amino acid mutation. Therefore, the resistance mechanism of Ae. albopictus to organophosphorus and carbamate insecticides needs to be further explored.
ZHU Cai-ying, ZHAO Chun-chun, LIU Qi-yong, LI Gui-chang, YAN Dong-ming, SONG Xiu-ping, WANG Jun, MENG Feng-xia
. Distribution of the ace gene of Aedes albopictus in different geographical populations in China[J]. Chinese Journal of Vector Biology and Control, 2020
, 31(2)
: 133
-136
.
DOI: 10.11853/j.issn.1003.8280.2020.02.003
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