Objective To investigate the epidemic process and molecular and epidemiological characteristics of the brucellosis in Menyuan county, Qinghai province, China, in April 2018, and to provide a reference for handling related events. Methods An on-site epidemiological investigation of the epidemic situation was performed using serological test methods (serum tube agglutination test and complement fixation test) and blood culture. The molecular characteristics of the strains were analyzed by multiple loci VNTR analysis (MLVA). Meanwhile, analyses were performed using patients'medical record data and laboratory test results. Results Two patients were infected with brucellosis due to contact with sheep with contagious abortion, and were diagnosed based on the Diagnostic Criteria for Brucellosis (WS269-2007) with acute stage of brucellosis according to their history of epidemiological exposure, clinical symptoms and signs, and laboratory test results. The two strains were genotype 42 as revealed by MLVA-8, and MLVA-16 genotyping proved the two strains to be 100% homologous. Conclusion MLVA can be used to analyze the association between areas with epidemic or sporadic brucellosis, trace the source of infection, and provide reliable technical support for trans-regional tracking and prevention and control of brucellosis.
ZHAO Zhong-zhi, XUE Hong-mei, ZHANG Ai-ping, XU Li-qing, MA Li, XIE Hui, ZHAO Yuan-bo, ZHAO Hong-yan, JIANG Hai, LI Ji-quan
. An investigational case report of brucellosis in Menyuan county, Qinghai, China[J]. Chinese Journal of Vector Biology and Control, 2020
, 31(1)
: 105
-107
.
DOI: 10.11853/j.issn.1003.8280.2020.01.022
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