Objective To investigate the insecticidal efficacy of common insecticides for bed bugs in the laboratory by bed bug bioassay, and to establish the median lethal dose (LD50) system for beg bugs. Methods Micro-topical application was used to determine the mortality rate of Cimex lectularius at different doses of common insecticides, and the probability unit regression method was used to calculate LD50 of different insecticides against bed bugs. Results The LD50 of thiamethoxam oxazine, imidacloprid, chlorpyrifos, dichlorvos, fenthion, λ-cyhalothrin, and fipronil for C. lectularius was 0.003 57, 0.000 91, 2.388 1, 1.934 7, 0.074 4, 9.370 7, and 0.081 47 μg/bug, respectively. As for insecticides with high level of resistance, the approximate value of LD50 was also preliminarily evaluated using pre-experiment, and the LD50 of these insecticides against C. lectularius was basically 0.5 μg/bug or above. Combined with the pre-experiment results of common pesticides for which LD50 had not been measured, it was inferred that all the common insecticides tested ranked as follows in terms of their insecticidal effect on bed bugs:nicotinoids, phenylpyrazoles, organophosphorus, pyrethroids, carbamates, and organic chlorides. Conclusion In the process of chemical control of bed bugs, nicotinoids and phenylpyrazoles, which are sensitive to bedbugs, should be the first choice, and the fenthion of organophosphorus is also a good choice.
JIA Xiao-kai, GAO Chun-hua, ZHOU Dan-dan, WEI Fu-rong, LIU Qi-yong, REN Dong-sheng
. Measurement of the median lethal dose of several common insecticides against Cimex lectularius[J]. Chinese Journal of Vector Biology and Control, 2020
, 31(1)
: 46
-48
.
DOI: 10.11853/j.issn.1003.8280.2020.01.010
[1] 汪诚信. 有害生物治理[M]. 北京:化学工业出版社,2005:468-471. Wang CX. Pest management[M]. Beijing:Chemical Industry Press,2005:468-471.
[2] 韩招久,王宗德,姜志宽,等. 萜类臭虫驱避剂筛选的初步研究[J]. 中华卫生杀虫药械,2013,19(5):383-385. Han ZJ,Wang ZD,Jiang ZK,et al. A preliminary study on Cimex lectularius repellents screening from terpenoids[J]. Chin J Hyg Insect Equip,2013,19(5):383-385.
[3] Goddard J,deShazo R. Bed bugs (Cimex lectularius) and clinical consequences of their bites[J]. JAMA,2009,301(13):1358-1366. DOI:1001/jama.2009.405.
[4] Doggett SL,Dwyer DE,Peñas PF,et al. Bed bugs:clinical relevance and control options[J]. Clin Microbiol Rev,2012,25(1):164-192. DOI:10.1128/CMR.05015-11.
[5] 郑剑宁,裘炯良. 臭虫防治研究进展[J]. 中华卫生杀虫药械,2011,17(2):145-148. Zheng JN,Qiu JL. Progress in bedbug control research[J]. Chin J Hyg Insect Equip,2011,17(2):145-148.
[6] 李兴文,马涛,王蓬,等. 深圳地区臭虫侵害调查研究[J]. 中华卫生杀虫药械,2013,19(3):236-238,241. Li XW,Ma T,Wang P,et al. Bug hazard investigation in Shenzhen[J]. Chin J Hyg Insect Equip,2013,19(3):236-238,241.
[7] WHOPES. Pesticides and their application:for the control of vectors and pests of public health importance[M]. 6th ed. Geneva:WHOPES,2006:62-63.
[8] Kolb A,Needham GR,Neyman KM,et al. Bedbugs[J]. Dermatol Ther,2009,22(4):347-352. DOI:10.1111/j.1529-8019.2009. 01246.x.
[9] 许荣满. 臭虫危害的复燃和防治[J]. 中华卫生杀虫药械,2010,16(5):398-399. Xu RM. Resurgence of bed bug hazards and control[J]. Chin J Hyg Insect Equip,2010,16(5):398-399.
[10] Wang CL,Wen XJ. Bed bug infestations and control practices in China:implications for fighting the global bed bug resurgence[J]. Insects,2011,2(2):83-95. DOI:10.3390/insects2020083.
[11] 任东升,吴海霞,郭玉红,等. 2018年全国臭虫监测报告[J]. 中国媒介生物学及控制杂志,2019,30(2):151-153. DOI:10.11853/j.issn.1003.8280.2019.02.008. Ren DS,Wu HX,Guo YH,et al. National vectors surveillance report on bed bugs in China,2018[J]. Chin J Vector Biol Control,2019,30(2):151-153. DOI:10.11853/j.issn.1003.8280.2019. 02.008.
[12] Romero A,Potter MF,Potter DA,et al. Insecticide resistance in the bed bug:a factor in the pest's sudden resurgence?[J]. J Med Entomol,2007,44(2):175-178. DOI:10.1093/jmedent/44. 2.175.
[13] 马世伟,刘小闪. 4种有机磷药物杀灭温带臭虫的实验研究[J]. 中国媒介生物学及控制杂志,2014,25(1):68-69. DOI:10.11853/j.issn.1003.4692.2014.01.019. Ma SW,Liu XS. Laboratory evaluation on efficacy of four organophosphates against bedbug Cimex lectularius[J]. Chin J Vector Biol Control,2014,25(1):68-69. DOI:10.11853/j.issn.1003.4692.2014.01.019.
[14] Romero A,Potter MF,Haynes KF. Behavioral responses of the bed bug to insecticide residues[J]. J Med Entomol,2009,46(1):51-57. DOI:10.1603/033.046.0107.