Objective To prevent the transmission of diseases by the breeding vectors and their carried pathogens on an entry international navigation container ship. Methods In October 2016, an entry international container ship seriously infested with vectors in the phase II dock of Nansha port in Guangdong, China was disinsectized based on the breeding status of vectors and the structure of ship. Sulfuryl fluoride fumigation was applied at a concentration of 8 g/m³ for 24 h in the cargo compartment and living areas. Cypermethrin insecticide spray (40 mg/m², 30 min) was applied twice (before unloading and before fumigation) in the areas where it was unsuitable for fumigation, such as main engine room, deck, storeroom, anchor chain room, and lifeboat. Results The mortality rates of test insects and test rodents, which were used to evaluate the effect of sulfuryl fluoride fumigation, were 100% after 24 h of sulfuryl fluoride fumigation. No living vectors were observed in the spray areas by vector monitoring before departure. Conclusion The effect of sulfuryl fluoride fumigation on the entry international navigation container ship is satisfying, providing a valuable experience for the fumigation of large international navigation ships.