Objective To perform whole-genome sequencing and phylogenetic analyses of Chikungunya virus (CHIKV) from the serum sample of a febrile patient who had traveled to Bangladesh in Quzhou, 2017. Methods Blood samples were collected aseptically from the patient. The serum was separated for detection of nucleic acids of CHIKV and Dengue virus by RT-PCR. The genome-wide target gene fragments of CHIKV were amplified by RT-PCR and then sequenced. The whole-genome sequence was analyzed by bioinformatic programs:Contig Express, SeqMan, Clustalx V2.0, BioEdit V7.0, GENEDO and MEGA X. Results The patient's serum was positive for CHIKV nucleic acids. The sequencing data were assembled to obtain the whole-genome sequence of CHIKV strain QZ0823, containing 11 787 bp nucleotides. No mutation was found in major structural sites of non-structural proteins. For structural proteins, E1 lacked the adaptive mutation A226V; E2 lacked the new substitution mutation K252Q that might affect the neurovirulence of CHIKV; there were no Aedes aegypti adaptive mutations E1:K211E or E2:V264A. The whole genome-based phylogenetic analysis showed that QZ0823 was tightly clustered with the epidemic strains in Bangladesh, India, and Pakistan, suggesting a close evolutionary relationship. Conclusion The nucleotide sequences of whole genome and structural genes C-E3-E2-6K-E1 of the imported virus in Quzhou show obvious features of CHIKV. QZ0823 and the epidemic strains in Bangladesh are derived from the Eastern, Central, and Southern African genetic lineage.
WANG Xiao-guang, YANG Rui-jun, HUANG Shi-teng, CAO Guo-ping, LEI Yong-liang, YE Ling
. Whole-genome characterization of imported Chikungunya virus in Quzhou, Zhejiang province, China, 2017[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(6)
: 621
-625
.
DOI: 10.11853/j.issn.1003.8280.2019.06.006
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