Objective To investigate the resistance of Rattus losea to the first-generation anticoagulant rodenticide in Xinhui district, Jiangmen, Guangdong province, China, and to elucidate the relationship between vitamin K epoxide reductase complex subunit 1 (VKORC1) gene mutation and rodenticide resistance. Methods The lethal feeding period test was used to determine the resistance of R. losea. The genomic DNAs of the rodents were extracted to clone the VKORC1 gene and screen out mutation and polymorphism sites. Meanwhile, the correlation between VKORC1 gene mutation and resistance was analyzed. Results The resistance rate of R. losea to the first-generation anticoagulant rodenticide was 27.03%, indicating the formation of a resistant population. The total length of the VKORC1 gene was 2 166 bp. A total of 12 mutation sites were detected and one of them was an insertion-deletion site. Exon 1 and exon 3 each had one mutation site, while intron 1 and intron 2 had six and four mutation sites, respectively. The exon 1 mutation resulted in an amino acid mutation at site 58 (Arg58Gly) and the exon 3 mutation was a synonymous mutation (Cys96Cys). A correlation analysis showed that there was no significant correlation between rodenticide resistance of R. losea and single nucleotide polymorphism site of the VKORC1 gene (P>0.05). Conclusion The site 58 amino acid mutation of the VKORC1 gene is not the main cause of rodenticide resistance in R. losea. The genetic mechanism of rodenticide resistance in R. losea may be related to other factors.
YAO Dan-dan, JIANG Hong-xue, LIU Fu-jia, FENG Zhi-yong
. A study of the resistance of Rattus losea to the first-generation anticoagulant rodenticide and its correlation with the VKORC1 gene[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(6)
: 613
-617
.
DOI: 10.11853/j.issn.1003.8280.2019.06.004
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