Objective To observe the effect of clarified lime water on Aedes albopictus at different stages of larval development in the laboratory and determine the effect of quicklime in the prevention and control of Ae. albopictus, and to provide basic data for the application of quicklime in the prevention and control of Ae. albopictus. Methods A certain number of Ae. albopictus at different developmental stages (eggs, 1 st to 4 th instar larvae, and pupae) were separately reared in clarified lime water, and the hatching rate, mortality rate, eclosion rate, and development time for each stage were recorded. Meanwhile, the development of Ae. albopictus in different feeding environments and different stages was analyzed and compared by taking the mosquitoes reared in overnight tap water as a control. Results The hatching rate of Ae. albopictus eggs in the experimental group was 82.22%, and that in the control group was 94.44%; the hatching rate of Ae. albopictus eggs was significantly lower in the experimental group than in the control group (χ²=6.523, P=0.011). According to the t-test, the hatching time of eggs was significantly longer in the experimental group than in the control group. The mortality rate of the 1st to 4th instar larvae was significantly higher in the experimental group than in the control group (χ²=4.624, 234.994, and 284.312, P=0.032, <0.003, and <0.003); particularly, the mortality rate of the 3rd and 4th instar larvae was significantly increased in the experimental group. There was no significant difference in eclosion rate between the experimental group and the control group (χ²=0.593, P=0.441). Conclusion Clarified lime water has a significant inhibitory effect on the development of Ae. albopictus, which increases with the age of mosquito larvae. The results imply that quicklime can be possibly used for the prevention and control of Ae. albopictus. The field control effect of quicklime on Ae. albopictus needs further verification.