Objective To analyze genetic mutations, predict the level of resistance to pyrethroid insecticides in the bedbug Cimex lectularius and provide a guide for bed bug control in this region. Methods Partial sequences of vgsc gene that encodes the target of pyrethroid insecticides (voltage gated sodium channel) were obtained by DNA sequencing, and the knockdown resistance-related mutations were examined. Results The six bed bugs collected on site were found to have genetic mutations at codons 419 and 925, leading to double amino acid substitutions (V419L and L925I) with a frequency of 100%. Conclusion All the investigated bed bugs carry kdr mutations at positions 419 and 925 of the voltage gated sodium channel, predicting that this bed bug population has a higher level of resistance to pyrethroid insecticides.
LIU Nian, ZHOU Xiao-jie, QI Guang-hao, LI Mei, QIU Xing-hui
. Detection of knockdown resistance mutations in a Cimex lectularius field population in Beijing, China[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(5)
: 524
-527
.
DOI: 10.11853/j.issn.1003.8280.2019.05.010
[1] Reinhardt K,Siva-Jothy MT. Biology of the bed bugs (Cimicidae)[J]. Ann Rev Entomol,2007,52:351-374. DOI:10.1146/annurev. ento.52.040306.133913.
[2] Wang CL,Singh N,Cooper RA,et al. Evaluation of an insecticide dust band treatment method for controlling bed bugs[J]. J Econ Entomol,2013,106(1):347-352. DOI:10.1603/EC12259.
[3] Susser SR,Perron S,Fournier M,et al. Mental health effects from urban bed bug infestation (Cimex lectularius L.):a cross-sectional study[J]. BMJ Open,2012,2(5):e000838. DOI:10.1136/bmjopen-2012-000838.
[4] Dong K,Du YZ,Rinkevich F,et al. Molecular biology of insect sodium channels and pyrethroid resistance[J]. Insect Biochem Mol Biol,2014,50:1-17. DOI:10.1016/j.ibmb.2014.03.012.
[5] Sayono S,Hidayati APN,Fahri S,et al. Distribution of voltage-gated sodium channel (Nav) alleles among the Aedes aegypti populations in central Java province and its association with resistance to pyrethroid insecticides[J]. PLoS One,2016,11(3):e0150577. DOI:10.1371/journal.pone.0150577.
[6] Dang K,Doggett SL,Singham GV,et al. Insecticide resistance and resistance mechanisms in bed bugs,Cimex spp. (Hemiptera:Cimicidae)[J]. Parasit Vectors,2017,10(1):318. DOI:10.1186/s13071-017-2232-3.
[7] Palenchar DJ,Gellatly KJ,Yoon KS,et al. Quantitative sequencing for the determination of kdr-type resistance allele (V419L,L925I,I936F) frequencies in common bed bug (Hemiptera:Cimicidae) populations collected from Israel[J]. J Med Entomol,2015,52(5):1018-1027. DOI:10.1093/jme/tjv103.
[8] Zhu F,Wigginton J,Romero A,et al. Widespread distribution of knockdown resistance mutations in the bed bug,Cimex lectularius (Hemiptera:Cimicidae),populations in the United States[J]. Arch Insect Biochem Physiol,2010,73(4):245-257. DOI:10.1002/arch.20355.
[9] Campbell BE,Koehler PG,Buss LJ,et al. Recent documentation of the tropical bed bug (Hemiptera:Cimicidae) in Florida since the common bed bug resurgence[J]. Fla Entomol,2016,99(3):549-551. DOI:10.1653/024.099.0333.
[10] 王陇德. 病媒生物防制实用指南[M]. 北京:人民卫生出版社,2010:94-96.
[11] Rinkevich FD,Zhang L,Hamm RL,et al. Frequencies of the pyrethroid resistance alleles of Vssc1 and CYP6D1 in house flies from the eastern United States[J]. Insect Mol Biol,2006,15(2):157-167. DOI:10.1111/j.1365-2583.2006.00620.x.
[12] Potter MF. The history of bed bug management-with lessons from the past[J]. Am Entomol,2011,57(1):14-25. DOI:10.1093/ae/57.1.14.
[13] Doggett SL,Russell RC. The resurgence of bed bugs,Cimex spp. (Hemiptera:Cimicidae) in Australia:experiences from down Under[C]//Proceedings of the 6th International Conference on Urban Pests. Pápai,Hungary:OOK Press,2008:407-425.
[14] Romero A,Potter MF,Potter DA,et al. Insecticide resistance in the bed bug:a factor in the pest's sudden resurgence?[J]. J Med Entomol,2007,44(2):175-178. DOI:10.1093/jmedent/44.2.175.
[15] Yoon KS,Kwon DH,Strycharz JP,et al. Biochemical and molecular analysis of deltamethrin resistance in the common bed bug (Hemiptera:Cimicidae)[J]. J Med Entomol,2008,45(6):1092-1101. DOI:10.1093/jmedent/45.6.1092.
[16] Dang K,Toi CS,Lilly DG,et al. Detection of knockdown resistance mutations in the common bed bug,Cimex lectularius (Hemiptera:Cimicidae),in Australia[J]. Pest Manag Sci,2015,71(1):914-922. DOI:10.1002/ps.3861.
