Objective To investigate the effect of ciprofloxacin hydrochloride on the content of energy substances in the larvae and pupae of Musca domestica. Methods From June to September, 2013, the laboratory population of M. domestica was used for the experiment. The newly hatched larvae were divided into control group (fed with normal forage) and three experimental groups (treated with forage containing 0.1%, 0.3%, and 0.5% ciprofloxacin hydrochloride, respectively). The grinding method was used for the sampling of energy substances in the 1st instar larvae, and hemolymph was collected from the 2nd and 3rd instar larvae and pupae. The Coomassie brilliant blue method was used to measure the content of proteins, anthrone-sulfuric acid colorimetry was used to measure the content of total sugars, and phosphoric acid-vanillin chromogenic reaction was used to measure the content of lipids. SPSS 20.0 software was used for statistical analysis; a one-way analysis of variance was used for comparison between groups; the t-test was used for comparison between two groups. Results The content of proteins in the larvae and pupae in the 0.5% ciprofloxacin hydrochloride group decreased to a maximum of 4.00% and 10.00% of that in the control group; the content of lipids in the larvae in the 0.1%, 0.3%, and 0.5% ciprofloxacin hydrochloride groups decreased to 75.00%, 65.00%, and 50.00% of that in the control group, while the content of lipids in the pupae in the 0.1%, 0.3%, and 0.5% groups decreased to 85.00%, 75.00%, and 50.00% of that in the control group; the content of total sugars in the 0.1%, 0.3%, and 0.5% groups decreased to 80%, 60%, and 30% of that in the control group. After the larvae and pupae of M. domestica were fed with ciprofloxacin hydrochloride, their caloric values decreased with the increase in the concentration of ciprofloxacin hydrochloride (F=12.551, P<0.001). The control group had higher caloric values of the larvae and pupae than the 0.1%, 0.3%, and 0.5% ciprofloxacin hydrochloride groups (t=13.093, 11.294, 8.601, all P<0.001); the 0.1% group had higher caloric values than the 0.3% and 0.5% ciprofloxacin hydrochloride groups (t=10.290, 7.842, all P<0.001); the 0.3% group had higher caloric values than the 0.5% group (t=6.919, P<0.001). Conclusion Ciprofloxacin hydrochloride can reduce the synthesis of energy substances (proteins, lipids, and total sugars) in the larvae and pupae of M. domestica and thus reduce their caloric values, thus effects the individual development and population multiplication of M. domestica.
WAN Qing, SONG Nuan, HUANG Zhen-dong, XUE Zhi-jing, ZHUANG Gui-fen, ZHANG Rui-ling, XU Yong-yu, ZHANG Zhong
. Effect of ciprofloxacin hydrochloride on energy substances in the larvae and pupae of Musca domestica[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(5)
: 514
-518
.
DOI: 10.11853/j.issn.1003.8280.2019.05.008
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