Objective To understand the living habits and facial Demodex infection of resident college students in a medical college in Wuhu city, and to provide advice for them to develop good hygienic habits. Methods One medical colleges and universities, in accordance with the principle of voluntary survey to Wuhu resident students using the scotch tape to paste the night method for creep mite etiology examination, and sets up a questionnaire on students' personal living habits, facial health situation, investigate the relevant factors such as diet, using Excel 2007 software for data collection, using SPSS 19.00 software for statistical analysis, The χ² test was used for the analysis of infection rate. Results A total of 120 questionnaires were issued and 113 valid questionnaires were returned. Among them, 33 cases were positive for facial Demodex infection, with a total infection rate of 29.20%. The infection rates of male and female students were 29.63% and 28.81%, respectively, and the difference was not statistically significant (χ²=0.009, P=0.924). The χ² test showed that the infection rate of Demodex was statistically significant (χ²=6.651, P=0.038) and it was high among those who ate greasy food. However, the infection rate of Demodex showed no significant difference between respondents with different lifestyles (all P>0.05). Conclusion Taste preference is related to the infection rate of facial demodex among resident students in the medical college in Wuhu. It is of great significance for the facial health of college students to urge them to develop good living and eating habits, which is conducive to the prevention and control of Demodex infection.