Objective To isolate culturable bacteria from the intestines of Blattella germanica, and to investigate the activity of digestive enzymes produced by these bacteria. Methods Traditional isolation and culture method was used for the isolation and purification of the bacteria from the intestines of B. germanica, and 16S rRNA sequencing was used for identification. The flat transparent circle method was used to screen out the bacteria producing amylase, cellulase, protease, or lipase, and the ratio of transparent circle diameter (D) to bacterial colony diameter (d) was used to compare the activities of digestive enzymes produced by different bacteria. SPSS 20.0 software was used for statistical analysis. A one-way analysis of variance was used for comparison between groups; the S-N-K multiple comparison method was used for comparison of data with homogeneity of variance, and the Dunnett's T3 method was used for the significance analysis of data with heterogeneity of variance. Results A total of 20 species of bacteria belonging to 12 genera were isolated from the intestines of B. germanica, among which there were 4 species in Enterococcus, 3 species in Bacillus, 3 species in Lysinibacillus, 2 species in Citrobacter, and 1 specie each in Serratia, Rhizobium, Pseudomonas, Morganella, Microbacterium, Enterobacterium, Chryseobacterium, and Ventosimonas. Among these bacteria, 7 produced protease and 3 produced amylase; no bacteria produced cellulase or lipase. The protease produced by S. marcescens and C. massiliae had relatively high activity, while that produced by M. oxydans, B. amyloliquefaciens, B. paramycoides, B. tequilensis, and P. aeruginosa had relatively low activity; there was a significant difference in the activity of produced protease between the 7 species of bacteria (F=8.859, P=0.000), while there was no significant difference in the activity of amylase produced by different bacteria (F=2.920, P=0.130). Conclusion Some bacteria in the intestines of B. germanica can produce protease and amylase, and such bacteria can assist food digestion in B. germanica.
HUNAG Zhen-dong, WAN Qing, XUE Zhi-jing, ZHANG Rui-ling, ZHANG Zhong
. Isolation and identification of culturable aerobic bacteria from the intestines of Blattella germanica and the activity of digestive enzymes produced by these bacteria[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(4)
: 409
-413
.
DOI: 10.11853/j.issn.1003.8280.2019.04.012
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