ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Effects of ciprofloxacin hydrochloride on the individual growth and development and population reproduction of Musca domestica
Received date: 2018-12-22
Online published: 2019-06-20
Supported by
Supported by the National Natural Science Foundation of China (No. 81572028, 81401693, 81271874)
Objective To investigate the effects of ciprofloxacin hydrochloride on the individual growth and development and population reproduction of Musca domestica. Methods The newly laid eggs of M. domestica were placed on the filter paper soaped with 0.1%, 0.3%, and 0.5% ciprofloxacin hydrochloride or sterile water (control). The hatching rates of the eggs were observed. The hatched larvae were fed with 0.1%, 0.3%, and 0.5% ciprofloxacin hydrochloride or without ciprofloxacin hydrochloride (control). The development of M. domestica was observed every day and the developmental durations of egg stage, larval stage, and pupal stage were calculated. The male and female adults emerged from ciprofloxacin pupae and control pupae were paired. The ciprofloxacin adults were fed with brown sugar (nutrients) and sterile water with 0.1%, 0.3%, and 0.5% ciprofloxacin, while the control adults were fed with brown sugar (nutrients) and sterile water (control). The pre-oviposition period, number of eggs laid per female, and lifetime of adults were calculated. The age-stage, two-sex life table software was used to calculate the age-stage survival rate, fertility, number of eggs laid per female, intrinsic rate of increase, finite rate of increase, and net reproductive rate of M. domestica and to evaluate the effects of ciprofloxacin hydrochloride on the individual growth and development and population reproduction of M. domestica. Statistical analysis was performed using SPSS 20.0 software. Comparison between groups was made by one-way analysis of variance and comparison between two independent samples was made by the t test. Results The developmental durations of egg stage and pupal stage in 0.1%, 0.3%, and 0.5% ciprofloxacin groups were 1 day and 6 days, respectively. There were no significant differences in the durations of egg stage and pupal stage between the three ciprofloxacin groups and the control group (all P>0.05). The duration of larval stage in the control group and the three ciprofloxacin groups were 5.80±0.09 days and 8.44±0.06 days, respectively, and there was a significant difference in the duration of larval stage between the control group and the three ciprofloxacin groups (F=40.927, P=0.000). The lifetime of female adults and male adults in the control group vs the three ciprofloxacin groups were 28.39±0.92 days vs 17.19±0.85 days and 26.27±1.12 days vs 17.31±0.98 days, respectively, and there were no significant differences in the lifetime of female adults and male adults between the control group and the three ciprofloxacin groups (female:F=20.091, P=0.000; male:F=14.218, P=0.000). The pre-oviposition period of female adults and the number of eggs laid per female in the control group vs the three ciprofloxacin groups were 4.94±0.10 days vs 6.37±0.33 days and 638.76±39.32 vs 137.37±24.22, respectively, and there was a significant difference between the control group and the three ciprofloxacin groups (F=28.336, P=0.000). The age-stage, two-sex life table study showed that the hatching rates of M. domestica eggs, mortality rates in the whole life cycle, life expectancy of newly laid eggs, and life expectancy of female adults in the control group vs the three ciprofloxacin groups were 96.43% vs 68.75%, 15.48% vs 46.09%, 36.07 days vs 18.03 days, and 32.03 days vs 19.88 days, respectively. From the perspective of population ecology, the intrinsic rate of increase, finite rate of increase, and net reproductive rate in the control group vs the three ciprofloxacin groups were 0.23 vs 0.13, 1.26 vs 1.14, and 258.55 vs 28.98, respectively. Conclusion After feeding with ciprofloxacin hydrochloride, the individual development of M. domestica is retarded, fertility is decreased, and population growth rate is significantly inhibited.
SONG Nuan, HUANG Zhen-dong, XUE Zhi-jing, WAN Qing, ZHUANG Gui-fen, ZHANG Rui-ling, XU Yong-yu, ZHANG Zhong . Effects of ciprofloxacin hydrochloride on the individual growth and development and population reproduction of Musca domestica[J]. Chinese Journal of Vector Biology and Control, 2019 , 30(3) : 300 -305 . DOI: 10.11853/j.issn.1003.8280.2019.03.016
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