Laboratory rearing and observation on the biological characteristics of Triatoma rubrofasciata

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  • National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China

Received date: 2018-12-28

  Online published: 2019-06-20

Supported by

Supported by the National Key Research and Development Plan (No. 2016YFC1202000, 2016YFC1202002) and National Science and Technology Major Project of China (No. 2018ZX10101002-005)

Abstract

Objective To establish a set of standard techniques and methods for laboratory rearing of Triatoma rubrofasciata, and to provide a reference for understanding the life cycle and molecular biological characteristics of T. rubrofasciata, the diseases transmitted by T. rubrofasciata, and the prevention and control of these diseases. Methods Triatoma rubrofasciatawas artificially fed in an incubator with a constant temperature of (28±1)℃ and a relative humidity of (70±5)% under the laboratory condition of light for 12 hours and darkness for 12 hours. Kunming rats were used as the sole blood-feeding animal. Results Triatoma rubrofasciatacompleted the whole life cycle (126-153 days) under the artificial rearing condition. Stage I-V nymphs and adults all were fed with the blood of rats. Conclusion The reproduction of T. rubrofasciata is successfully carried out by laboratory rearing.

Cite this article

ZHU Dan, LIU Qin, ZHANG Yi, LI Yuan-yuan, WU Jia-tong . Laboratory rearing and observation on the biological characteristics of Triatoma rubrofasciata[J]. Chinese Journal of Vector Biology and Control, 2019 , 30(3) : 281 -285 . DOI: 10.11853/j.issn.1003.8280.2019.03.012

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