Objective To investigate the resistance of Musca domestica to six hygienic insecticides in Jinnan district, Tianjin, China, to explore the pattern of changes in the resistance of M. domestica to insecticides in Jinnan district by comparing that to the results monitored in 2014 and taking into account the history of use of insecticides in Jinnan district, and to provide a scientific basis for the prevention and control of M. domestica. Methods In August, 2017, M. domestica was collected from agricultural markets, livestock farms, and dump sites in Jinnan district, Tianjin. The micro-titration method was used to determine the resistance of M. domestica to insecticides. The laboratory results were statistically analyzed using DPS 7.05; a regression curve for virulence, median lethal dose (LD₅₀), and 95% confidence interval (CI) were calculated. Results The resistance of M. domestica to six insecticides was observed in Jinnan district, with an upward trend in the resistance found in four comparable insecticides (propoxur, beta-cypermethrin, permethrin, and deltamethrin) as mentioned above. The resistance to propoxur was highest with a LD₅₀ of >15 μg/♀ and a resistance ratio of >352.94. The LD₅₀ for beta-cypermethrin, permethrin, deltamethrin, lambda-cyhalothrin, and pirimiphos-methyl was 0.054 8, 0.108 0, 0.095 1, 0.064 3, and 0.743 6 μg/♀, respectively, with the corresponding resistance ratios as 91.33, 9.64, 158.50, 20.09, and 11.05, compared with the susceptible strains reported in the literature. Compared with the results monitored in 2014, the resistance levels were on the rise, with the resistance ratios to propoxur, beta-cypermethrin, permethrin, and deltamethrin as >12.60, 14.81, 9.15, and 3.37, respectively. Conclusion Musca domestica in Jinnan district have developed a resistance to various insecticides with a rising trend in resistance. Sustainable prevention and control strategies aiming at environmental and physical control are recommended. It is advisable to choose low-resistance chemical insecticides based on the insecticide resistance monitoring results, and to avoid using the same insecticide against M. domestica for a long period of time.