Objective To establish a DNA barcoding method for identification of common species of acaroid mites. Methods From March to December, 2017, acaroid mites were collected from flour, Chinese medicinal materials, and normal diet in different parts of Anhui province, China, for morphological identification. Gene sequences of mitochondrial cytochrome C oxidase subunitⅠ(COⅠ) from corresponding species were used as standard fragments. The COⅠ gene sequences from known acaroid mites were collected from the National Center for Biotechnology Information and analyzed using DNAStar and MEGA 6.0. A phylogenetic tree was constructed using the neighbor-joining (NJ) algorithm. Results Morphological identification revealed 7 species of acaroid mites:Acarus siro, Aleuroglyphus ovatus, Tyrophagus putrescentiae, Lepidoglyphus destructor, Gohieria fusca, Chortoglyphus arcuatus, and Dermatophagoides farinae. The results of DNA barcoding identification were consistent with the morphological results. The interspecific genetic distance was 0.058-0.323, while the intraspecific genetic distance was 0.000-0.015. In the NJ phylogenetic tree, the cluster analysis results of acaroid mites were consistent with the taxonomic status by morphological identification. Conclusion DNA barcoding can be used as a supplement to morphological identification, which provides fast and accurate identification of the species of acaroid mites.
ZHENG Ling-xiao, YIN Can-can, WANG Yi-xiao, LI Meng-nan, SHAO Huang-fang, FANG Wei-xi, YANG Man, ZHAO Jin-hong, SUN En-tao
. Identification of acaroid mite species based on DNA barcoding[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(2)
: 180
-184
.
DOI: 10.11853/j.issn.1003.8280.2019.02.015
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