Objective To investigate the current status of vectors infestation in the urban area of Wuhan, China, in 2017, and to provide a scientific basis for vector control and National Hygienic City construction. Methods From June to September, 2017, a field investigation was performed to evaluate the current status of the vectors infestation in different types of units (places). The rodent trace method was used for the survey of rodents; the path method and the larva dip method were used for the survey of mosquitoes; visual observation was used for the survey of flies and cockroaches, and of the investigation of facilities for the prevention of rodents and flies. Results The total positive rate of indoor rodent density was 10.1%, the total failure rate of rodent-poof facilities was 17.2%, and the total path index of outdoor rodent density was 3.4. The total path index of water in small containers was 4.3, and the total dip index of large and medium waters was 5.8%. The density of mosquito larvae per positive dip was 5.5. The total positive rate of indoor adult flies was 7.3%; the total failure rate of fly-poof facilities was 14.0%; the total positive rate of fly breeding sites was 3.0%. The total invasion rate of indoor adult nymphae was 4.1%; the total positive rate of oothecae was 1.2%; the total positive rate of cockroach trace was 4.6%. Conclusion The current status of the vectors infestation in different types of units (places) of Wuhan obtained in this investigation provides background data for effective vector control and the consolidation of National Hygienic City in the future.