Objective To compare different laboratory assays for identifying imported oval malaria in laboratories and to analyze the genotypes, and to avoid misdiagnosis and missed diagnosis of oval malaria. Methods Five cases of imported oval malaria returning from labor in Liaoning province, China were diagnosed by three methods:Giemsa staining microscopy, combined Biotech malaria RDT diagnostic kit, and nested PCR, and the results were compared. The 18S rRNA genes of Plasmodium falciparum in peripheral blood samples from the five cases were subjected to sequencing and homology analysis. Results The morphology of typical P. ovale was observed in all samples by Giemsa staining microscopy. The results of RDT detection and PCR detection of malaria were all positive. The 18S rRNA gene of Plasmodium falciparum was sequenced and a sequence of 800 bp in length was obtained. The homology of the 18S rRNA with that in oval malaria was up to 99% by GenBank alignment. Conclusion The results of nested PCR detection, rapid detection, and staining microscopy for oval malaria cases are consistent (P. ovale). It is necessary to promptly strengthen the ability of microscopic examination for Plasmodium in the epidemic areas of malaria, and its combination with molecular biological detection technology can greatly reduce misdiagnosis and missed diagnosis of Plasmodium in laboratories.
WANG Bo, MAO Ling-ling, WANG Zi-jiang, LI Xin, YU Wei-jun, SONG Ge, WANG Zhou-chao, SUN Ying-wei
. Imported oval malaria in Liaoning province, China, in 2017: a laboratory diagnosis of 5 cases[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(1)
: 80
-83
.
DOI: 10.11853/j.issn.1003.8280.2019.01.019
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