Objective To investigate the genotypes and genetic characteristics of Yersinia pestis strains isolated from Kangbao county in Hebei province, China in 2017. Methods The DNA of three Y. pestis strains isolated from Kangbao county in Hebei province in 2017 was extracted by boiling method and kit extraction method. According to the literature, the primers of 22 different regions (DFRs) and pMT1 were synthesized and the DFR sites were amplified by PCR. The genotype was analyzed after agarose gel electrophoresis. Results The DFRs in the three strains were DFR02-05, DFR08-11, DFR14, DFR19-22, and pMT1, but not DFR01, 06, 07, 12, 13, and 15-18. The genotype was G20 after alignment. Conclusion The genotype of Y. pestis isolated from the plague foci in Hebei province in 2017 is G20 and different from G17 isolated previously. The emergence of new genotype provides a basis for further study of the genetic characteristics and epidemiological characteristics of the strain.
WANG Hai-feng, ZHOU Song, LIU He-zhi, LIU Xiao-wei, YANG Shun-lin, DU Guo-yi, SHI Xian-ming
. Genotypes and epidemiological characteristics of three Yersinia pestis strains isolated from Kangbao county in Hebei province, in 2017[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(1)
: 40
-42
.
DOI: 10.11853/j.issn.1003.8280.2019.01.008
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