Objective To investigate the etiological characteristics and epidemiological significance of Yersinia pestis strains in Haibei Tibetan Autonomous Prefecture, Qinghai province, and to provide a scientific basis for the prevention and control of plague in this area. Methods Carbohydrate fermentation test, virulence factor detection, virulence determination, plasmid analysis, and different region (DFR) analysis were performed on a total of 104 Y. pestis strains isolated from Qilian, Menyuan, Haiyan, and Gangcha in Haibei Tibetan Autonomous Prefecture from 1956 to 2011. Results According to the biochemical typing, 73 of the 104 strains tested were classified as the Qinghai-Tibet Plateau ecotype, 25 as the Qilian Mountain ecotype, and 6 as the ecotypes different from those in the natural plague foci of Qinghai province. Plasmids with molecular weights of 6×106, 45×106, and 52×106 were found in all of the strains. Seventy-seven (74.04%) of the 104 strains contained four virulence factors, and 98.51% (66/67) were velogenic strains. According to the DFR analysis, six genomovars were found:genomovar 8 (75 strains), genomovar 44 (20 strains), genomovar 5 (6 strains), genomovar 6 (1 strain), genomovar 7 (1 strain), and genomovar 11 (1 strain). Conclusion The Y. pestis strains isolated in Haibei Tibetan Autonomous Prefecture, Qinghai province have the specific characteristics of Y. pestis strains in Qinghai-Tibet plateau, with complex ecotypes and strong virulence. Monitoring, prevention, and control of plague, as well as dissemination of relative knowledge, should be strengthened in this area.
WU Hai-sheng, WU Hai-lian, YANG Xiao-yan, LI Cun-xiang, XIONG Hao-ming, HE Jian, LI Xiang, XU Xiao-qing, DAI Rui-xia, ZHANG Qing-wen
. An etiological study and epidemiological significance of Yersinia pestis in Haibei Tibetan Autonomous Prefecture, Qinghai province, China[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(1)
: 35
-39
.
DOI: 10.11853/j.issn.1003.8280.2019.01.007
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