Objective To investigate the prevalence of 6 rodent-borne pathogens, in some suburban areas of Beijing, China. Methods TaqMan fluorescent probe-based quantitative real-time PCR (qPCR) was used for laboratory evaluation. Plasmid standards were prepared and standard curves were plotted. Detection thresholds were determined according to the reevaluated data. From January to June, 2017, the trap-at-night method was used to capture rodents in Chaoyang, Huairou, Mentougou, and Pinggu districts of Beijing, tissue samples were collected to extract nucleic acids, and qPCR was used to detect pathogens. Results A total of 160 rodents were captured, among which there were 59 Rattus norvegicus rodents, 69 Mus musculus rodents, 4 Apodemus peninsulae rodents, 25 Niviventer confucianus, and 3 Sorex spp.. The examination of spleen tissues showed that all rodents except Sorex were infected with Rickettsia typhi (8.1%), Orientia tsutsugamushi (1.3%), Anaplasma phagocytophilum (5.6%), Leptospira interrogans (18.7%), or Bartonella spp. (5.0%). No Francisella tularensis was detected in all rodents. There were co-infections with various pathogens including Leptospira interrogans and A. phagocytophilum in R. norvegicus and M. musculus. Conclusion Rodents in Beijing carry the pathogens of common, emerging and reemerging infectious diseases. The distribution investigation of animal hosts and vectors of these pathogens in these areas and prevention and treatment of related infectious diseases should be strengthened.
LI Dong-mei, XU Zhao-nan, ZHU Cai-ying, ZHANG Zheng, KANG Yang, ZHANG Hong-jiang, SONG Xiu-ping, TANG Cheng-jun, GE Jun-qi
. An epidemiologic investigation of rodent-borne pathogens in some suburban areas of Beijing, China[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(1)
: 12
-17
.
DOI: 10.11853/j.issn.1003.8280.2019.01.003
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