Objective Survey the resistance trend of Culex pipiens pallens to Permethrin, Deltamethrin, beta-Cypermethrin, Fenobucarb, and Temephos in Kaifeng and Anyang, to provide scientific guidance for chemical prevention. Methods Larvae were collected from flower gardens or abandoned pools and so on in the urban area of Kaifeng and Anyang from June to August in 2012, 2014, and 2016. Results The median lethal concentration (LC₅₀) of the Kaifeng population against Permethrin in 2012, 2014, 2016 were 0.147 5-0.523 8 mg/L, the resistance ratios were 5.76-20.46 fold; against Deltamethrin were 0.006 2-0.011 7 mg/L, the resistance ratios were 31.00-58.50 fold; against beta-Cypermethrin, Fenobucarb and Temephos were 0.001 6-0.513 4 mg/L, the resistance ratios were 0.22-7.90 fold. The LC₅₀ of the Anyang population against Permethrin, beta-Cypermethrin, Fenobucarb and Temephos were 0.000 8-0.915 5 mg/L, the resistance ratios were 0.50-8.00 fold; against Deltamethrin were 0.002 2-0.012 2 mg/L, the resistance ratios were 11.00-61.00 fold. Conclusion Culex pipiens pallens in Kaifeng and Anyang showed different levels of resistance to pyrethroids and organophosphorus insecticides, but still susceptible to carbamate insecticides. The strategy of mosquito control in Kaifeng and Anyang should focus on source reduction. Chemical control should be limited, scientific and effective.