Objective To investigate the breeding situation of flies in different environments in Wuhan and to provide scientific evidence for fly control. Methods To investigate the breeding situation of flies in different environment in Wuhan from May to October 2016 by visual method. The fly larval breeding rates between different months were compared with χ² test. Results A total of 5 094 breeding sites were found, and there were 320 fly larval breeding sites, with a mean fly larval breeding rate of 6.3%. The fly larval breeding rate in different environs ranged from 0 to 16.8%. In the demolition site, the fly larval breeding rate was the highest, accounted for 16.8%, followed by the refuse transfer station 13.5%, the public greenbelt 12.9%, the construction site 12.6%, the railway line 12.4%, the farm produce market 8.9%, the real estate district 7.9%. The breeding rate of fly larvae in food processing plants was the lowest, i.e. 0. The fly larval breeding rate in May was the highest, accounted for 7.8%, and lowest in October, accounted for 5.7% (χ²=2.967, P=0.085). Conclusion To master the breeding situation of flies in different environments of each type of habitat in Wuhan city, to provide good background information for the prevention and control of flies in the future, as well as disease vector management in hygienic city campaign.