Objective To analyze the genotypes and genetic characteristics of Yersinia pestis isolated from Hebei plague foci. Methods The DNA of Y. pestis collected in Kangbao county from 1972 to 2017 was extracted by boiling method, the multiple locus variable number tandem repeat (MLVA) and the DFR primers according to a publication were synthesized by a biological company, then amplified by polymerase chain reaction, then genotypes were analyzed by agarose gel electrophoresis. Results All of the strains showed one MLVA genotype, and the main genotypes of Y. pestis from Hebei foci were G17 and G20. The absence of DFRs were 1, 6, 7, 13, 15, 16, 17, 18 in G17, the absence of DFRs were 1, 6, 7, 12, 13, 15, 16, 17, 18 in G20. Conclusion The genetic characteristics of enzootic plague in Hebei province remain stable. Further studies are warranted to confirm whether the epidemics among wildlife in 2017 was caused by spread of neighboring foci or by genetic shifting of the pathogen.
WANG Hai-feng, ZHANG Yi-hui, YANG Xiao-yan, LIU Yi-yang, NIU Yan-fen, LIU Guang
. Studies on the genotypes of Yersinia pestis in Hebei province by two genotyping methods[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(6)
: 564
-566
.
DOI: 10.11853/j.issn.1003.8280.2018.06.004
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