Objective To provide scientific foundation for proper disposition and control efficacy against bedbug infestation in passenger trains by a case study of such. Methods Visual inspection was conducted to investigate the bedbug infestation in a passenger train and control efficacy was assessed. The beddings in positive carriages were washed using hot water (over 60℃). The carriage was treated by lambda-cyhalothrin microcapsule suspensions when the train was in operation, and by beta-cypermethrin and Phoxim EC when the carriage was uncoupled. The control efficacy was observed on the 3^rd, the 6^th and the 12^th month post-treatment. Results In total, 147 bedbugs were found before insecticide treatment, counts declined to 21 on the 3^rd day (85.71% reduction), 6 on the 6^th day (95.92% reduction), and 0 on the 15^th day (100% reduction). After the first time emergency disposition, there was no subsequent bedbug biting incidence. The bedbug wasn't found within 1 year after treatment. Conclusion While managing the beddings well, pyrethroids can be used for preventing bedbug infestations. Organophosphorus pesticides can also be used for thorough control the bedbugs when the carriage is uncoupled.