Objective To identify Yersinia pestis from the Mongolian gerbils and EV76 bacteria of plague by different region (DFR) fragment genotyping. Methods The DNA of 6 strains of Y. pestis and EV76 isolates was extracted by boiling method from Hangjinqi of Inner Mongolia in 2015, Yinchuan city of Ningxia Hui Autonomous Region in 2013, and Kangbao county of Hebei province in 2003. The DFR primers according to a report were synthesized by biological company, then amplified by PCR, analyzed genotype through agarose gel electrophoresis. Results The DFR genotypes of Y. pestis from Mongolian gerbils were G11, G17, and G20. The absent points of G11 were DFR01, 06, 07, 13, 15, 16, and 17; DFR18 was also absent for G17 in addition to the above; The absent point of G20 also included DFR12 in addition to G17. The absence DFRs of EV76 were 01, 02, 04, and 10. Conclusion Using the differential fragment genotyping method can quickly distinguish Y. pestis isolates from Mongolian gerbils and EV76. At the same time, it is possible to trace the source of the epidemic rapidly by identifying the genotype of wild strains.
WANG Hai-feng, ZHOU Song, LI Yu-gui, BAI Xue-wei, SUN Rui, HU Le-le
. Identification of Yersinia pestis isolates from Mongolian gerbils and the plague vaccine via genotyping of different regions[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(5)
: 436
-438
.
DOI: 10.11853/j.issn.1003.8280.2018.05.003
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