Objective To investigate the distribution patterns of mosquitoes and mosquito-borne viruses in northeast Guizhou province. Methods In 2017, host-seeking mosquitoes were collected from livestock sheds using Ultraviolet lights in Dejiang and Jiangkou, Guizhou province. The mosquito specimens were classified according to morphology and stored in liquid nitrogen. All the samples were incubated with BHK-21 and C6/36 cells for virus isolation and detected the virus genes. The virus isolates were identified and analyzed by molecular biological methods. Results In total, there were 7 380 mosquitoes from 3 genera and 3 species collected from Dejiang and Jiangkou, Guizhou province, 61.55% (4 542/7 380) of which were Armigeres subalbatus and 26.80% (1 978/7 380) were Anopheles sinensis. Out of the 3 virus strains isolated from the mosquitoes, One (GZDJ1765) was identified as typeⅠof Japanese encephalitis virus (JEV) isolated from Culex tritaeniorhynchus and two (GZDJ1746-1, GZDJ1752-1) were identified as Getah virus isolated from A. subalbatus. Three mosquito pools (GZDJ1704, GZDJ1743-2, GZDJ1751-2) tested positive for JEV genes y by PCR. Conclusion Armigeres subalbatus was the predominant species in northeast Guizhou. JEV and GETV were isolated.
SONG Song, FU Shi-hong, LI Yuan-yuan, LI Xiao-long, WANG Ding-ming, TIAN Zhen-zao, ZHOU Jing-zhu, HE Ying, LEI Wen-wen, WANG Huan-yu, WANG Bin, LU Xiao-qing, LIANG Guo-dong
. Investigation of mosquitoes and mosquito-borne viruses in Guizhou province, 2017[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(5)
: 428
-435
.
DOI: 10.11853/j.issn.1003.8280.2018.05.002
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