Objective Nested PCR and quantitative real-time PCR were applied to identify the infection of Borrelia burgdorferi in rodents in this study, so that application of these two methods in host surveillance of Lyme disease can be evaluated. Methods The rodents were collected from Guertu, Xinjiang Uygur Autonomous Region during July to September in 2017. The rodents were examined for the presence of B. burgdorferi by nested PCR and real-time PCR. Results In total of 134 rodent samples, the positive rate by nested PCR was 13.43% with 18 positive samples identified, whereas 17 samples were identified positive by qRT-PCR assay and the positive rate was 12.69%. The cycle threshold(Ct) value was 33.49-37.89. There was no significant difference between the two assays (χ2=0.000, P=1.000). All the positives were detected from Spermophilus undulatus. Conclusion The results suggested that both nested PCR and real-time PCR could be used in identifying B. burgdorferi in rodents. Combination of these two assays could increase the positive rate. The results of nested PCR could provide local-predominant genotypes; however bacterial loads can be estimated by qRT-PCR assay.
ZHANG Lin, MIAO Guang-qing, HOU Xue-xia, LI Bo, HAO Qin
. Evaluation of nested PCR and real-time PCR in host surveillance of Lyme disease[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(5)
: 425
-427
.
DOI: 10.11853/j.issn.1003.8280.2018.05.001
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