Objective To investigate the prevalence and diversity of spotted fever group rickettsia infection(SFGR) in ticks in northeastern China. Methods Flagging for free-living ticks and off-host(cattle and sheep) collection for parasitic ticks were conducted from 9 counties and Heixiazi Island from Northeastern China, during May to June, 2012-2014. Conventional PCR was carried out using universal SFGR primers targeting ompA genes to screen for their infection with SFG rickettsia organisms in ticks collected. The positive products were sequenced, and molecular phylogenetic tree was established. Results The presence of SFGR was 32 of 280 pools(1 224 ticks) tested, with overall positive rate 11.43%. There were no significant differences in different regions(χ2=7.683, P=0.566) and in different tick species(χ2=6.354, P=0.174) for their infection with SFGR rickettsia. The phylogenetic analysis based on nucleotide sequence showed that 14 SFGR DNA sequences belonged to 3 SFGR species:5 samples from Dermacentor silvarum, 1 from each of Haemaphysalis concinna and H. japonica were clustered together with Rickettsia heilongjiangensis 054(AF179362.2) and HL-93(AF179364.1), showing 99.30%-100% identity; 3 samples from H. longicornis were clustered with Candidatus R. hebeiii(QHD-3.HQ651817.1, TS-1.HQ651818.1, QHD-1.HQ651815.1) with 99.83%-100% homogeny; 4 samples from D. silvarum were clustered together with R. sp. DnS14(AF009130.2), and Jilin Strain R. sp. JL-02(AY093696.1) with 98.79%-100% identity. Conclusion The prevalence of SFGR in ticks from Northeastern China was high. There was high diversity in SFG rickettsia species and tick species in the areas surveyed.
WANG Zhuo, WANG Jian-wei, YU Miao, XING Yue-peng, FENG Li, YANG Yi-jun, WANG Xiu-hong, TIAN Pu, WU Yi-min
. Molecular epidemiological studies on spotted fever group rickettsia in ticks from Northeastern China[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(4)
: 344
-347
.
DOI: 10.11853/j.issn.1003.8280.2018.04.005
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