Identification and traceability of an intercepted tick from imported dog at Shanghai port

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  • Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China

Received date: 2018-02-24

  Online published: 2018-08-20

Supported by

Supported by the National Key Research and Development Program of China(No. 2016YFC1202000, 2016YFC1202004)

Abstract

Objective To identify the unknown species of ticks carried by the dog imported from Germany to China. Methods Use morphological identification, and extract the genomic DNA from the ticks, amplify the mitochondrial 16S rRNA sequences and sequencing. Blast in the NCBI, to identify the species of the ticks collected from Shanghai port on November 28, 2016. Results Morphological analysis revealed that the tick was in accordance with the morphological characteristics of Haemaphysalis. The sequence analysis results showed that the measured gene of this tick was 98% identical to the mitochondrial gene sequence of Ha. flava Neumann, 1897 which was widely distributed in Palaearctic and Oriental region. Conclusion This tick is Ha. flava, and infected the dog after entry.

Cite this article

WANG Qiang, HE Yu-ping, WU Cheng-yun, WANG Qiao-quan, WANG Yan, LIN Ying-zheng, CHEN Zhi-fei, ZHANG Qiang, DENG Yao-hua, ZHANG Guan-nan, CAO Yu-fan, TANG Zhi-fang, LI Shu-qing . Identification and traceability of an intercepted tick from imported dog at Shanghai port[J]. Chinese Journal of Vector Biology and Control, 2018 , 29(4) : 340 -343 . DOI: 10.11853/j.issn.1003.8280.2018.04.004

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