Objective To provide scientific evidence for integrated control of Musca domestica, study the synergism of Nasonia vitripennis and Beauveria bassiana. Methods The "Y" olfactometer and host-selection tests were used to research the selectivity of female N. vitripennis to M. domestica pupae exposed to B. bassiana spores. Ecological simulation test was used to investigate the effects of B. bassiana on the reproductive potential and life-span of emerged M. domestica adults after being exposed to the spores at pupae stage. Difference of measurement data was compared with single factor analysis of variance using SPSS package. Results There was preference by female N. vitripennis to B. bassiana spores-treated M. domestica pupae and controls. The pre-oviposition period of M. domestica adults emerged from B. bassiana spores-treated pupae and controls were about(6.00±0.94) and(6.07±0.78) d, respectively, there were no statistical significant differences between them. The oviposition of female M. domestica adults emerged from B. bassiana spores-treated pupae and controls were(152.20) 14.37) and(633.17) 17.08) eggs, respectively. The hatchability of eggs ovipositted by flies treated with spores at pupae stage and controls were about(20.64±1.74)% and(88.63±2.05)%. The life span of female flies emerged from spore-exposed pupae and controls were about(4.93±1.74) and(28.93±2.70) d, respectively. So the female fecundity and egg hatchability of M. domestica treated with B. bassiana spores at pupae stage were reduced to 1/4 of controls, life span of female flies emerged from spore-exposed pupae dropped to 1/6 of controls, the reproductive potential of female flies emerged from spore-treated pupae dropped to 1% of control groups. Conclusion Beauveria bassiana spore treatment had no significant effects on the parasitic rate of female N. vitripennis to M. domestica pupae and the pre-oviposition period of emerged female flies. In the breeding grounds of M. domestica, releasing N. vitripennis could control 80% of the pupae. Spraying B. bassiana spores combined with releasing N. vitripennis could reduce the reproductive potential of M. domestica by 99%. The combined method is an effective means to the integrated biological control of M. domestica.
HUANG Zhen-dong, WAN Qing, ZHUANG Gui-fen, XUE Zhi-jing, ZHANG Rui-ling, ZHANG Zhong
. Synergestic control efficacy of Nasonia vitripennis and Beauveria bassiana against Musca domestica[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(4)
: 336
-339
.
DOI: 10.11853/j.issn.1003.8280.2018.04.003
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