Objective To investigate the natural infection with Dengue virus(DENV) and Japanese encephalitis virus (JEV) in mosquitoes in Jinghong city, Xishuangbanna prefecture, Yunnan province, China, and provide the scientific basis for its prevention and control. Methods Mosquito samples were collected from urban area of Jinghong in August and September 2015. Mosquito samples were tested for the nucleic acid of DENV and JEV by RT-PCR assay, and positive specimens were sequenced for C/PrM gene for homology and phylogenetic analyses. Results A total of 2 009 mosquitoes were collected in urban area of Jinghong. Aedes albopictus, Ae. aegypti, Culex(species not identified) and Ae. vexans were 896, 477, 634, and 2 of the total, respectively. The JEV RNA were detected from one pool of Culex mosquitoes and one pool of Ae. albopictus mosquitoes. The sequences of C/PrM genes of the 2 JEV strains were obtained. Homology and phylogenetic analyses showed that the 2 JEV strains are consistent with the genotypeⅠ(G-Ⅰ) and its clade of the same G-Ⅰ strains of JEV from GenBank, and the JEV strains from Jinghong were closely related to the JEV G-Ⅰ strains from Honghe prefecture of Yunnan in 2009, Dehong prefecture of Yunnan in 2010 and Gansu province, China in 2008. Mosquito samples tested negative for DENV in the study. Conclusion Existence of JEV G-Ⅰ in Jinghong city of Xishuangbanna prefecture was firstly confirmed from mosquitoes in 2015. It should be strengthened to monitor and control Japanese encephalitis epidemics in this area.
PAN Hong, GAO Yang, FENG Yun, HAN Xi, ZHANG Jing, ZHU Jin, LI Wei-ping, LI Hong-bin, FAN Jian-hua, ZHANG Hai-lin
. Identification of Japanese encephalitis viral infections in mosquitoes in Jinghong city, Yunnan province, China[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(4)
: 331
-335
.
DOI: 10.11853/j.issn.1003.8280.2018.04.002
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